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<p style="text-align: justify;">Optimization of carrageenan production from Persian Gulf algae <em>Laurencia obtusa</em>: Purification and rheological characterization</p>

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Title Statement <p style="text-align: justify;">Optimization of carrageenan production from Persian Gulf algae <em>Laurencia obtusa</em>: Purification and rheological characterization</p>
 
Added Entry - Uncontrolled Name Entezari, Tayebeh ; Department of Biological Science and Technology, Faculty of Nano and Bio Science and Technology, Gas and Petrochemical Engineering, Persian Gulf University, Bushehr 75169, Iran
Zarei, Mandana ; Department of Biological Science and Technology, Faculty of Nano and Bio Science and Technology, Gas and Petrochemical Engineering, Persian Gulf University, Bushehr 75169, Iran
Azari, Ahmad ; Department of Chemical Engineering, Faculty of Petroleum, Gas and Petrochemical Engineering, Persian Gulf University, Bushehr 75169, Iran
Iranian Vice-Presidency for Science and Technology (Grant number: 11/78299).
 
Uncontrolled Index Term Barcoding; COI; Design-Expert; RbcL; Rheometric analysis
 
Summary, etc. <p style="text-align: justify;">In this study, <em>Laurencia obtusa </em>(Yamada, 1931) species was investigated for carrageenan extraction. <em>L. obtusa </em>species was identified using the molecular barcoding method with two COI and rbcL genes. Rheological analysis was performed to determine the rheological properties of semi-refined carrageenans. Experimental design was carried out with Design Expert software to produce and optimize SRC extraction. The software presented 18 treatments based on temperature, cooking time and concentration of KOH. Treatments were prepared for rheometric analysis (i.e., viscosity measurements). Optimization was performed by the software with maximum viscosity. Also, two refined carrageenan extraction methods were performed, and the FTIR and NMR spectra were compared. Barcoding showed similarity values of 96 and 94% using the COI sequence and therbcL sequence, respectively. The optimal treatment included temperature of 77°C, 99 minutes, and 7% concentration of w/v KOH. The highest efficiency was associated with the dialysis method, which was 19%. Based on the rheometric analysis, the SRC solution was introduced as a pseudo plastic Non-Newtonian fluid. According to these two analyses, the refined carrageenans of <em>L. obtusa </em>were of the iota type.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2022-12-05 09:59:16
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/45637
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 13, ##issue.no## 4 (2022): December 2022 (Ahead of Print)
 
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