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ATP-Bioluminescence Assay for Real Time Surface Hygiene Monitoring in Fish Value Chain

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Title ATP-Bioluminescence Assay for Real Time Surface Hygiene Monitoring in Fish Value Chain
Not Available
 
Creator Femeena Hassan
Geethalakshmi, V.
 
Subject Adenosine triphosphate-bioluminescence
hygiene monitoring
principle component analysis
safety and quality
value chain
 
Description Not Available
ATP-bioluminescence (ATP-B) assay was used to
evaluate organic debris load, which in turn indicates
the cleanliness of fish contact surfaces (FCS) in fish
value chain, where the last point is ready to eat
(RTE) fish. Contaminated FCS can harbour lot of
microorganisms including pathogens and specific
spoilage organisms. From the stipulated four critical
points in the value chain viz., harvesting unit
(coracle), auction cum assortment area (AAA), preprocessing
area (PPA), cooking and marketing area
(CMA). Specific FCS were selected for real time
surface hygiene monitoring using ATP bioluminescence
assay both following normal cleaning process
(NCP) and prescribed Sanitation Standard Operating
Procedures (SSOP). The present study has
significance in fish industry for monitoring hygiene
and establishing high risk zones in value chain, as
it gives real time information about hygiene once
critical limits were located after a justification
period. Visual assessment of cleanliness is unreliable
in monitoring surface hygiene in fish value chain,
and ATP-B testing can aid in the design, execution
and substantiation of effectual surface cleaning. For
successful monitoring and validation of surface
cleaning, a scheme that integrates visual judgment
and ATP-B testing, with periodical conventional
microbial swab testing is anticipated. The principal
component analysis illustratively divided the ATP
bioluminescence values of FCS following normal
cleaning process (NCP) and prescribed Sanitation
Standard Operating Procedures (SSOP).
Not Available
 
Date 2022-12-21T11:15:15Z
2022-12-21T11:15:15Z
2022-10
 
Type Research Paper
 
Identifier Femeena Hassan and Geethalakshmi, V. (2022) ATP-Bioluminescence Assay for Real Time Surface Hygiene Monitoring in Fish Value Chain. Fish. Techol. 59(4): 293-302.
0015-3001
http://krishi.icar.gov.in/jspui/handle/123456789/75240
 
Language English
 
Relation Not Available;
 
Publisher Society of Fishery Technologists India