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Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach

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Title Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
 
Creator Sandhya Sharma,
Anshika Tyagi,
Harsha Srivastava,
G. Ramakrishna,
Priya Sharma,
Amitha Mithra Sevanthi,
Amolkumar U. Solanke,
Ramavtar Sharma,
Nagendra Kumar Singh,
Tilak Raj Sharma
Kishor Gaikwad
 
Subject Galactomannan
RGC-936,
WRKY
 
Description Galactomannan is a polymer of high economic importance and is extracted from the seed endosperm of clusterbean (C. tetragonoloba). In the present study, we worked to reveal the stage-specific galactomannan biosynthesis and its regulation in clusterbean. Combined electron microscopy and biochemical analysis revealed high protein and gum content in RGC-936, while high oil bodies and low gum content in M-83. A comparative transcriptome study was performed between RGC-936 (high gum) and M-83 (low gum) varieties at three developmental stages viz. 25, 39, and 50 days after flowering (DAF). Total 209,525, 375,595 and 255,401 unigenes were found at 25, 39 and 50 DAF respectively. Differentially expressed genes (DEGs) analysis indicated a total of 5147 shared unigenes between the two genotypes. Overall expression levels of transcripts at 39DAF were higher than 50DAF and 25DAF. Besides, 691 (RGC-936) and 188 (M-83) candidate unigenes that encode for enzymes involved in the biosynthesis of galactomannan were identified and analyzed, and 15 key enzyme genes were experimentally validated by quantitative Real-Time PCR. Transcription factor (TF) WRKY was observed to be co-expressed with key genes of galactomannan biosynthesis at 39DAF. We conclude that WRKY might be a potential biotechnological target (subject to functional validation) for developing high gum content varieties.
ICAR-CRP on Genomics (NBFGR, Lucknow)
 
Date 2021-08-27T09:37:35Z
2021-08-27T09:37:35Z
2021-02-17
 
Type Research Paper
 
Identifier Sharma, S., Tyagi, A., Srivastava, H. et al. Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach. Sci Rep 11, 4000 (2021)
2045-2322
http://krishi.icar.gov.in/jspui/handle/123456789/60849
 
Language English
 
Relation Article number: 4000 (2021);
 
Publisher Nature Research