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Genetic Diversity in Punica granatum Revealed by Nuclear rRNA, Internal Transcribed Spacer and RAPD Polymorphism

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Title Genetic Diversity in Punica granatum Revealed by Nuclear rRNA, Internal Transcribed Spacer and RAPD Polymorphism
Not Available
 
Creator S. K. Singh
P. R. Meghwal
Rakesh Pathak
Ragini Gautam
Suresh Kumar
 
Subject Genetic diversity
Pomegranate
Punica granatum
RAPD
rRNA gene diversity
 
Description Not Available
The morpho-physiological and molecular
markers were used to reveal genetic diversity among 13
putative varieties of Punica granatum grown in India. A
hybrid protocol of DNA isolation was developed to obtain
high molecular weight quality genomic DNA. Ten RAPD
primers generated 119 marker bands amounting to 92.44 %
polymorphism with 88 % polymorphic information content.
The 5.8S rRNA gene region was found to be highly
conserved (99.34 %) followed by ITS-1 (96.58 %) and
ITS-2 (89.21 %) intron regions encompassing the gene.
ITS-2 region was recorded with the highest percentage of
parsimony informative sites with high divergence mainly
due to SNPs. The PCA accounted for 84.21 % of total
variance. The AMOVA of RAPD data revealed the maximum
genetic variation within population rather than among
populations. Nei’s gene diversity (h) was 0.2684 and
Shannon information index (i) was 0.4135. The Nei’s
genetic distance ranged from 0.0620 to 0.0878. The coefficient
of gene differentiation between populations (Gst) of
0.2584 and gene flow (Nm) of 1.4347 validates that the
main proportion of genetic variation was within
populations. The present study validates the utility of ITS
rDNA region as a reliable indicator of phylogenetic interrelationships,
especially ITS-2 as probable DNA barcode at
higher levels and can serve as an additional approach for
genetic cataloguing of pomegranate germplasm for crop
improvement.
Not Available
 
Date 2019-05-15T11:59:29Z
2019-05-15T11:59:29Z
2013-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19562
 
Language English
 
Relation Not Available;
 
Publisher Not Available