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Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India

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Title Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India
Not Available
 
Creator Ragini Gautam
S. K. Singh
Vinay Sharma
 
Subject RAPD
ITS
Fusarium
Neocosmospora
Syncephalastrum
 
Description Not Available
The productivity of arid legumes, such as Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna
unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum), may remain stagnant
over decades because of their high susceptibility to root diseases. Besides, there is a limitation on the information about molecular diagnosis and intraspecific genetic variability of root pathogens in arid legumes. To
contribute in this field, we assessed a total of 52 isolates from 88 root samples that were found infected with
fungal pathogens in Jodhpur, Jaipur and Bikaner Districts of Rajasthan. Diseased roots samples were analyzed
following standard microbiological methods for fungus extraction and purification, and for genetic studies.
Irrespective of the geographical location from where the diseased samples were collected, all pathogen isolates
were clustered in RAPD dendrograms as per their respective genera. Phylogram, based on multiple sequence
alignment, revealed that different genera (i.e. Fusarium, Neocosmospora and Syncephalastrum), separated from each other, and species within the same genera, clustered together with their reference sequences with apreciable bootstrap values. Out of 20 representative isolates representing each cluster and all outgroups sequenced, eight were molecularly identified as Neocosmospora vasinfecta, five as Fusarium solani, two as Neocosmospora striata, two as Fusarium acutatum, one as Syncephalastrum monosporum, one as Fusarium oxysporum and
one as Fusarium species. The root pathogens of the arid legumes were found neither restricted to a geographical location nor were host specific in nature. Fusarium solani wilt in cowpea and seedling rot in moth bean, F. oxysporum wilt in moth bean, F. acutatum damping off in cowpea and Clusterbean, Fusarium sp. seedling rot in Clusterbean, Neocosmospora striata root rot in cowpea and wilt in Clusterbean and Syncephalastrum monosporum root rot in Clusterbean were molecularly identified as new fungal records as pathogens causing root diseases in arid legumes. Rev. Biol. Trop. 64 (4): 1505-1518. Epub 2016 December 01.
Not Available
 
Date 2019-05-15T11:54:53Z
2019-05-15T11:54:53Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19557
 
Language English
 
Relation Not Available;
 
Publisher Not Available