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Molecular markers of Ryadg gene and serological assay reveal potato virus Y (PVY) resistance in the tetraploid Indian potato (Solanum tuberosum) germplasm

Indian Agricultural Research Journals

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Title Molecular markers of Ryadg gene and serological assay reveal potato virus Y (PVY) resistance in the tetraploid Indian potato (Solanum tuberosum) germplasm
 
Creator TIWARI, JAGESH K
PANDEY, S K
POONAM, POONAM
CHAKRABARTI, S K
GOPAL, J
KUMAR, VINOD
 
Subject ELISA, Molecular markers, PVY resistance, Ryadg gene, Solanum tuberosum
 
Description The Ryadg gene derived from Solanum tuberosum ssp andigena provides extreme resistance to PVY. ThePVY resistant potato germplasms tested previously through ELISA were used in present experiment for examining presence/absence of Ryadg gene through linked molecular markers. SCAR and CAPS markers were used as a tool for selecting resistant clones in early generation, to determine the source of resistance and validate the earlier results. Fifty four potato germplasms were screened with molecular markers and by ELISA after mechanical inoculation with PVYO isolate. Eight germplasms validated PVY resistance by containing Ry,adg, gene and negative absorbance value (A405 nm) of ELISA as well. On the contrary, 39 germplasms werefound resistant through ELISA but did not show Ryadg gene. Therefore these 39 resistant germplasms would be the future research materials to identify source of resistance through different molecular markers from wider genetic base of wild potatoes species. Thus, our results demonstrate the application of Ryadg gene linked molecular markers as early, rapid and more reliable technique for the screening of PVY resistance in potato germplasms as an alternative to ELISA.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-04-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/28838
 
Source The Indian Journal of Agricultural Sciences; Vol 83, No 4 (2013)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/28838/13044
 
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