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PCR detection of Ralstonia solanacearum: A new approach for rapid detection of bacterium from infected plants

Indian Agricultural Research Journals

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Title PCR detection of Ralstonia solanacearum: A new approach for rapid detection of bacterium from infected plants
 
Creator DUTTA, RAM
BANERJEE, AMRITA
BEHERE, GAJANAN T
DEVI, K JINA
CHANDRA, SATISH
NGACHAN, S V
 
Subject Detective, PCR, Ralstonia solanacearum, Rapid Method
 
Description Ralstonia solanacearum (Smith) is a soil-borne plant pathogen responsible for causing bacterial wilt and having wide host range which includes monocots, dicots, annual plants/trees and shrubs. It is a most destructive disease of solanceaous crops and ginger in north eastern region of India. The pathogen is primarily present in soils as saprophytic bacterium and it has ability to survive for long periods of time in various natural habitats. The bacterium causes sudden wilting in plants and difficult to detect at the initial level as similar symptom may also occur with many fungal organisms like Fusarium spp. and Verticillium spp. An attempt was made to develop a PCR-based rapid method fordetection of this pathogen. This method requires only 3-5 hours against the conventional methods which generally require minimum 3 days to detect the pathogen. The PCR uses previously reported primer pairs for fliC gene (Rsol_fliC), which amplify 400bp region of fliC gene. The bacterial ooze from infected tissues was directly used as a source of DNA. The amplified product was cloned and sequenced for confirmation. The PCR based method developed in this report is very simple, robust and inexpensive and was successfully tested on four infected samples and further validated on over 50 samples of tomato which were infected by R. solanacearum.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2015-08-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/50815
 
Source The Indian Journal of Agricultural Sciences; Vol 85, No 8 (2015)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/50815/21624
 
Rights Copyright (c) 2015 The Indian Journal of Agricultural Sciences