Phenol test and electrophoresis of seed esterases for testing genetic purity in seed lots of pearl milllet (Pennisetum glaucum) inbreds
Indian Agricultural Research Journals
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Title |
Phenol test and electrophoresis of seed esterases for testing genetic purity in seed lots of pearl milllet (Pennisetum glaucum) inbreds
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Creator |
Varier, Anuradha
Dadlani, Malavika Sharma, S P |
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Subject |
—
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Description |
An experiment was conducted during 1993 to explore possibility of using phenol test and electrophoresis of seed esterases fortesting the genetic purity of seed lots or pearl millet [Pennisetum glaucume (L.) Br. emend. Stuntz.] inbreds. Ten inbredlines of pearlmillet (ICMB 87001', 'ICMB 87003', 'ICMB 88004', 'ICMB 88006', 'ICMB 89111', 'ICMB 90111', '841 B', '81 B','843 B' and '5141 B') could be grouped into 4 different colour categories using phenol test. Contamination of a seed lot onlywith seed of a different colour category could be detected by this technique. Polyacrylamide gel electrophoresis of seedesterases could differentiate each of these lines individually. This test could detect an artificial contamination of 4% (2seeds of the inbred '81 B' in 50 seeds or the line 'ICMB 90111'), indicating its potential for testing the genetic purity orpearl millet seed lots.
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Publisher |
The Indian Journal of Agricultural Sciences
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Contributor |
—
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Date |
2012-06-06
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
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Format |
application/pdf
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Identifier |
http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/19108
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Source |
The Indian Journal of Agricultural Sciences; Vol 65, No 11 (1995)
0019-5022 |
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Language |
eng
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Relation |
http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/19108/9451
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Rights |
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
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