Record Details

Cytochemical analysis and stain development in pollen grains of parental lines and hybrids based on cytoplasmic genetic male sterility system in pigeonpea (Cajanus cajan)

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Cytochemical analysis and stain development in pollen grains of parental lines and hybrids based on cytoplasmic genetic male sterility system in pigeonpea (Cajanus cajan)
 
Creator SINGH, F
SINGH, I P
MAJUMDER, N D
 
Subject Cajanus cajan; Cytoplasmic genetic male sterility; Pollen grains; Staining
 
Description A study was conducted during 2006–08 to determine viability of pollen grains in cytoplasmic genetic male sterile lines of pigeonpea [Cajanus Cajan (L.) Millsp.]. Poor pollen grain formation and their degeneration in subsequent mitotic cell division were evident in male sterile lines. Application of various specific stains revealed the following: carmine, which is used very often for assessing pollen viability may not be very reliable because it is non-specific nucleus, cytoplasm and other organelles, (ii) iodine + potassium iodide (I2 + KI) stain can precisely distinguish the fertile from sterile pollen grains and can also differentiate pollen grains in terms of chemical composition, and (iii) the mixed stain (carmine + ferrous ammonium sulphate + haematoxyline) is very specific to fertile vs sterile pollen grains and can identify various pollen grains with more precision. Staining technique can also be used to detect the distortions in respiratory mechanism and nucleic acid synthesis. Further, the under sized pollen grains and their frequency significantly varied among the ‘A’, ‘B’, ‘R’ lines and the hybrids synthesized there from.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-01-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2491
 
Source The Indian Journal of Agricultural Sciences; Vol 79, No 9 (2009)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2491/625
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences