Eicosapentaenoic Acid Enrichment from Sardine Oil by Argentation Chromatography
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Title |
Eicosapentaenoic Acid Enrichment from Sardine Oil by Argentation Chromatography Not Available |
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Creator |
Chakraborty,Kajal
Devakumar,C |
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Subject |
Sardine oil
eicosapentaenoic acid (EPA) fatty acid methyl esters (FAME) urea fractionation argentation column chromatography |
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Description |
Not Available
Eicosapentaenoic acid (EPA) derived from chemically hydrolyzed sardine oil was concentrated by urea fractionation using methanol at different temperatures (2, 4, and 6 °C) and urea/fatty acid ratios (2:1, 3:1, and 4:1 w/w) and purified by argentation neutral alumina column chromatography. The individual fatty acids were determined as fatty acid methyl esters (FAME) by gas-liquid chromatography and gas chromatography-mass spectroscopy as FAME and N-acyl pyrrolidides. In the mass fragmentation pattern of FAME, the base peak was assigned to be the 1-methoxyethenol moiety (m/ z) 74 obtained by McLafferty rearrangement. Formation of the cyclic tropylium ion (m/ z) 91 in fatty acids with four or more double bonds was apparent in FAME-PUFAs. The base peak of N-acyl pyrrolidides was the McLafferty rearrangement ion, 1-(pyrrolidin-1-yl)ethenol (m/ z) 113. The highest concentration of EPA (47.78%) was obtained at the crystallization temperature of 4 °C with a urea/fatty acid ratio of 4:1 (w/w) with 93.74% yield. After complexation of saturated and less unsaturated fatty acids by urea complexation, argentation chromatography resulted in an EPA of high purity (99.6%) with an overall recovery of 54.09% using 50% diethyl ether/ n-hexane as eluting solvent. The peroxide (POV) and thiobarbituric acid (TBS) values were found to be highest (4.0 mequiv of O2/kg and 5.2 mg of malondialdehyde/kg, respectively) during urea fractionation at the higher crystallization temperature (6 °C) and higher urea/fatty acid ratio (4:1). Not Available |
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Date |
2021-09-08T08:20:55Z
2021-09-08T08:20:55Z 2007 |
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Type |
Article
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/61818 |
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Language |
English
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Relation |
Not Available
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Publisher |
American Chemical Society
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