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Eicosapentaenoic Acid Enrichment from Sardine Oil by Argentation Chromatography

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Title Eicosapentaenoic Acid Enrichment from Sardine Oil by
Argentation Chromatography
Not Available
 
Creator Chakraborty,Kajal
Devakumar,C
 
Subject Sardine oil
eicosapentaenoic acid (EPA)
fatty acid methyl esters (FAME)
urea fractionation
argentation column chromatography
 
Description Not Available
Eicosapentaenoic acid (EPA) derived from chemically hydrolyzed sardine oil was concentrated by
urea fractionation using methanol at different temperatures (2, 4, and 6 °C) and urea/fatty acid ratios
(2:1, 3:1, and 4:1 w/w) and purified by argentation neutral alumina column chromatography. The
individual fatty acids were determined as fatty acid methyl esters (FAME) by gas-liquid chromatography
and gas chromatography-mass spectroscopy as FAME and N-acyl pyrrolidides. In the mass
fragmentation pattern of FAME, the base peak was assigned to be the 1-methoxyethenol moiety
(m/ z) 74 obtained by McLafferty rearrangement. Formation of the cyclic tropylium ion (m/ z) 91
in fatty acids with four or more double bonds was apparent in FAME-PUFAs. The base peak of
N-acyl pyrrolidides was the McLafferty rearrangement ion, 1-(pyrrolidin-1-yl)ethenol (m/ z) 113. The
highest concentration of EPA (47.78%) was obtained at the crystallization temperature of 4 °C with
a urea/fatty acid ratio of 4:1 (w/w) with 93.74% yield. After complexation of saturated and less
unsaturated fatty acids by urea complexation, argentation chromatography resulted in an EPA of
high purity (99.6%) with an overall recovery of 54.09% using 50% diethyl ether/ n-hexane as eluting
solvent. The peroxide (POV) and thiobarbituric acid (TBS) values were found to be highest (4.0 mequiv
of O2/kg and 5.2 mg of malondialdehyde/kg, respectively) during urea fractionation at the higher
crystallization temperature (6 °C) and higher urea/fatty acid ratio (4:1).
Not Available
 
Date 2021-09-08T08:20:55Z
2021-09-08T08:20:55Z
2007
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/61818
 
Language English
 
Relation Not Available
 
Publisher American Chemical Society