Keratinase mediated fabrication and partial characterization of gold nanoparticles and its antibacterial potential
Online Publishing @ NISCAIR
View Archive InfoField | Value | |
Authentication Code |
dc |
|
Title Statement |
Keratinase mediated fabrication and partial characterization of gold nanoparticles and its antibacterial potential |
|
Added Entry - Uncontrolled Name |
Sudha, Balraj Athinath, KS Swabna, Vivek Sumathi, Sundaravadivelu |
|
Uncontrolled Index Term |
Antibacterial activity; Antioxidant activity; Bio-nano synthesis; Waste utilization |
|
Summary, etc. |
Keratinase is mainly involved in recycling of keratin waste. Of late, researchers extended its application to nanotechnology. In the present study, we have made an attempt to fabricate and characterize gold nanoparticles using crude keratinase enzyme from Serratia ficaria and also study their biological application, particularly antibacterial activity. The formation of gold nanoparticles (AuNPs) was first verified by UV-Visible Spectroscopy. FTIR spectra confirmed the presence of responsible secondary metabolites for stabilization of nanoparticles. The morphological characteristics and particle size of synthesized nanoparticles were analyzed. The AuNPs showed significant antibacterial activity against Klebsiella pneumonia, Bacillus cereus and Staphylococcus aureus. The highest radical scavenging activity, 60.62% for AuNPs was observed at 500 µg/mL. Results of this study reveals significance of keratinase application in nano-based biological applications. |
|
Publication, Distribution, Etc. |
Indian Journal of Experimental Biology (IJEB) 2022-12-26 14:38:15 |
|
Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJEB/article/view/51286 |
|
Data Source Entry |
Indian Journal of Experimental Biology (IJEB); ##issue.vol## 61, ##issue.no## 01 (2023): IJEB [JANUARY 2023] |
|
Language Note |
en |
|