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Isolation and Characterization of Pomegranate (Punica granatum L.) Endophytes

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Title Isolation and Characterization of Pomegranate (Punica granatum L.) Endophytes
symposium on endophytes and their applications in agriculture
 
Creator Saranaya R.
Yashoda R. Hegde
 
Subject Pomegranate
Endophyte
Fungi
Bacteria
Characterization
 
Description Not Available
Endophytic fungi and bacteria were isolated from leaf and shoot of apparently healthy pomegranate plants. The plant samples were collected from 5 different districts of northern Karnataka which includes Bagalkote, Belagavi, Gadag, Koppal and Vijayapura. Totally 101 endophyes were isolated using standard isolation technique. Among them 69 were fungal endophyes (31 from leaf and 38 from shoot), and 38 were bacterial endophytes (18 from leaf and 14 from shoot). Cultural characters of fungal and bacterial endophyes were studied on potato dextrose agar and nutrient agar medium respectively. Among the 69 fungal isolates, growth characters of colony varied from white to grey and black on upper surface and pale yellow to light brown and black on lower surface, regular to irregular margin, fluffy to flat mycelial growth and smooth to coarse texture on PDA. Colony diameter ranged from 2.4 to 8.5 cm but maximum number of isolates showed above 8.0 cm colony diameter after ten days of incubation. The growth of isolates on nutrient agar medium showed considerable differences with respect to colony colour, appearance (form), elevation and margin. Colony colour varied from dull white to bright yellowish, circular to irregular form, flat to raised elevation and undulated to entire margin. Further these endophytes were evaluated against pomegranate wilt pathogens like Ceratocystis fimbriata and Fusarium oxysporum.
ICAR-National Agricultural Higher Education Project (NAHEP), New Delhi
University of Agricultural Sciences, Bengaluru
 
Date 2020-12-21T06:34:38Z
2020-12-21T06:34:38Z
2019-09-24
 
Type Other
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/43586
 
Language English
 
Publisher Not Available