Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii
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Title |
Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii
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Creator |
Manchi Rajesh
Biju Sam Kamalam Alexander Ciji Md. Shabaz Akhtar Debajit Sarma Narrotam Prasad Sahu Atul Kumar Singh |
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Subject |
Snow trout, elongation factor-1 alpha, house-keeping gene, reference gene, protein translation
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Description |
Not Available
In this manuscript, we report the cloning and in silico characterization of elongation factor 1A in a coldwater Trans-Himalayan cyprinid, Schizothorax richardsonii. The sequenced elongation factor 1A (SrEF1A) consisted of 1290 bp long partial ORF, which included a start codon (ATG) and 430 deduced amino acids. The SrEF1A sequence showed high homology (≥90%) with other teleosts and higher vertebrates. Phylogenetic analysis, multiple sequence alignment and prediction of conserved residues indicated a close evolutionary relationship among the cyprinids and conservation of SrEF1A protein across the vertebrate class. The deduced SrEF1A protein did not contain any signal peptide, but had two potential N-glycosylation motifs at 284th and 314th amino acid residue. Presence of many serine, threonine and tyrosine phosphorylation sites was also predicted, suggesting a potential post-translational regulation of the SrEF1A protein. In silico predictions of sub-cellular localization, function and protein-protein network illustrate the role of SrEF1A in the protein synthesis machinery of the cell. Finally, a reliable tertiary structure of SrEF1A protein was predicted with ten helixes and nineteen beta sheets. Ligand (GDP) binding sites in the tertiary structure were predicted at 15-22, 154, 156, 157 and 194-196 amino acid residues. Indian Council of Agricultural Research (ICAR) |
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Date |
2020-10-01T07:18:18Z
2020-10-01T07:18:18Z 2018-01-01 |
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Type |
Research Paper
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Identifier |
Rajesh, M., Kamalam, B.S., Ciji, A., Akhtar, M.S., Sarma, D., Sahu, N.P. and Singh, A.K., 2018. Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii. Journal of Entomology and Zoology Studies, 6(4): 1444-1453.
2320-7078 http://krishi.icar.gov.in/jspui/handle/123456789/41695 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Akinik Publications
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