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Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii

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Title Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii
Not Available
 
Creator Manchi Rajesh
Biju Sam Kamalam
Alexander Ciji
Md. Shabaz Akhtar
Debajit Sarma
Narrotam Prasad Sahu
Atul Kumar Singh
 
Subject Snow trout, elongation factor-1 alpha, house-keeping gene, reference gene, protein translation
 
Description Not Available
In this manuscript, we report the cloning and in silico characterization of elongation factor 1A in a coldwater Trans-Himalayan cyprinid, Schizothorax richardsonii. The sequenced elongation factor 1A (SrEF1A) consisted of 1290 bp long partial ORF, which included a start codon (ATG) and 430 deduced amino acids. The SrEF1A sequence showed high homology (≥90%) with other teleosts and higher vertebrates. Phylogenetic analysis, multiple sequence alignment and prediction of conserved residues indicated a close evolutionary relationship among the cyprinids and conservation of SrEF1A protein across the vertebrate class. The deduced SrEF1A protein did not contain any signal peptide, but had two potential N-glycosylation motifs at 284th and 314th amino acid residue. Presence of many serine, threonine and tyrosine phosphorylation sites was also predicted, suggesting a potential post-translational regulation of the SrEF1A protein. In silico predictions of sub-cellular localization, function and protein-protein network illustrate the role of SrEF1A in the protein synthesis machinery of the cell. Finally, a reliable tertiary structure of SrEF1A protein was predicted with ten helixes and nineteen beta sheets. Ligand (GDP) binding sites in the tertiary structure were predicted at 15-22, 154, 156, 157 and 194-196 amino acid residues.
Indian Council of Agricultural Research (ICAR)
 
Date 2020-10-01T07:18:18Z
2020-10-01T07:18:18Z
2018-01-01
 
Type Research Paper
 
Identifier Rajesh, M., Kamalam, B.S., Ciji, A., Akhtar, M.S., Sarma, D., Sahu, N.P. and Singh, A.K., 2018. Molecular cloning and in silico analysis of elongation factor 1A in Schizothorax richardsonii. Journal of Entomology and Zoology Studies, 6(4): 1444-1453.
2320-7078
http://krishi.icar.gov.in/jspui/handle/123456789/41695
 
Language English
 
Relation Not Available;
 
Publisher Akinik Publications