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Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822)

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Title Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinus mrigala (Hamilton 1822)
Not Available
 
Creator Snatashree Mohanty
Makesh, M.
Rajendran , K.V.
Suresh Babu
Deepika Anand
Saurav Kumar
Abhay Kumar
Raju Baitha
Kamal Sarma
 
Subject Cirrhinus mrigala
ELISA
Immunoglobulin
Monoclonal Antibodies
Western blotting
 
Description Not Available
Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purifed by affnity chromatography using BSA-CL agarose column. The purifed mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purifed m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purifed m-Ig indicated that, all the three MAbs were specifc to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps.
Not Available
 
Date 2021-03-04T06:12:34Z
2021-03-04T06:12:34Z
2020-04
 
Type Research Paper
 
Identifier Snatashree Mohanty, Makesh, M., Rajendran , K.V., Suresh Babu, Deepika Anand, Saurav Kumar, Abhay Kumar, Raju Baitha and Kamal Sarma (2020) Production and characterisation of monoclonal antibodies against immunoglobulins of Cirrhinusmrigala (Hamilton 1822) Indian J. Fish., 67(2): 55-61.
0970-6011
http://krishi.icar.gov.in/jspui/handle/123456789/45804
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research