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Evaluation of Culture Media for Selective Enrichment and Isolation of Salmonella in Seafood

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Title Evaluation of Culture Media for Selective Enrichment and Isolation of Salmonella in Seafood
Not Available
 
Creator Rakesh Kumar
Surendran, P. K.
Nirmala Thampuran
 
Subject Not Available
 
Description Not Available
Seafood, including fish, shrimp, clam, crab, mussel, oyster, lobster, squid, octopus, and cuttlefish samples, was used to compare the recovery of Salmonella serovars by different selective enrichment and isolation media. The samples were selectively enriched in Rappaport-Vassiliadis (RV) broth and tetrathionate broth (TT), followed by selective isolation on Hektoen enteric (HE) agar, xylose lysine desoxycholate (XLD) agar, bismuth sulfite (BS) agar, and Brilliant Green (BG) agar media. Of 443 seafood samples analyzed, 108 were found to be contaminated with Salmonella. The role of selective enrichment in Salmonella spp. recovery with RV medium was distinctly high (70%) compared to TT broth (30%). The selective enrichment in RV broth followed by selective isolation on XLD, HE, BS, and BG agar recovered Salmonella at levels of 56, 41, 28, and 16%, respectively. Similarly, after enrichment in TT broth, XLD and HE agars recovered 27 and 23 respectively. The recovery of Salmonella with enrichment in TT followed by isolation on BS and BG was abysmally low at 4.6 and 5, respectively. There was no significant difference (P > 0.05) in the recovery of Salmonella using the combinations of XLD and HE media with selective enrichment in RV broth. However, performance difference (P
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Date 2021-07-20T08:59:41Z
2021-07-20T08:59:41Z
2010-09-01
 
Type Research Paper
 
Identifier Rakesh Kumar, Surendran, P.K. and Nirmala Thampuran (2010) Evaluation of culture media forselective enrichment and isolation of Salmonella inseafood, J. AOAC Intl., 93(5): 1468-1471.
1060-3271
http://krishi.icar.gov.in/jspui/handle/123456789/49133
 
Language English
 
Relation Not Available;
 
Publisher Oxford Academic