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Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)

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Title Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822)
Not Available
 
Creator Abhishek Awasthi
Gaurav Rathore
Neeraj Sood
M. Y. Khan
W. S. Lakra
 
Subject Labeo rohita
Cell line
Macrophage
Peritoneal cavity
Phagocytosis
 
Description Not Available
A continuous leukocyte cell line with phagocytic activity was established from peritoneal macrophages of rohu, Labeo rohita (LRPM). LRPM was initiated from adherent mononuclear leukocytes isolated from peritoneal cavity of rohu, without use of any growth factors or feeder cells. These cells exhibited maximum growth at 30 C in L-15 medium containing 20 % foetal bovine serum, and has been subcultured for more than 60 passages till date. The cells showed 85 % viability after 6 months of storage in liquid nitrogen. The species of origin of the LRPM was confirmed by the amplification and sequencing of 655 bp fragment of cytochrome oxidase subunit I of mitochondrial DNA. Functionally, LRPM showed phagocytic activity of yeast cells and fluorescent latex beads as evaluated by phase contrast and scanning electron microscopy, respectively. Immuno-modulators such as bacterial lipopolysaccharide and phorbol myristate acetate resulted in functional activation of LRPM; and enhanced their microbicidal activity through release of reactive oxygen species and nitric oxide. Culture supernatant from activated cells also revealed lysozyme activity. Cells of LRPM were positive for alphanaphthyl acetate esterase enzyme indicating macrophage lineage. Our results indicate that this cell line can be a useful in vitro tool to study the role of macrophages in teleost immune system and to evaluate the effects of new aquaculture drugs. The LRPM cell line represents the first reported leukocyte cell line of peritoneal origin from any freshwater species of fish.
Not Available
 
Date 2021-07-19T09:53:07Z
2021-07-19T09:53:07Z
2013-11-19
 
Type Research Paper
 
Identifier Awasthi, A., Rathore, G., Sood, N., Khan, M.Y. and Lakra, W.S., 2015. Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822). Cytotechnology, 67(1), pp.85-96.
1573-0778
0920-9069
http://krishi.icar.gov.in/jspui/handle/123456789/48830
 
Language English
 
Relation Not Available;
 
Publisher Springer