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Digoxigenin-Labeled Probe-Based Colony Blotting Assay for Rapid Quantification of Salmonella Serovars in Seafood and Water

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Title Digoxigenin-Labeled Probe-Based Colony Blotting Assay for Rapid Quantification of Salmonella Serovars in Seafood and Water
Not Available
 
Creator Rakesh Kumar
Lalitha, K. V.
 
Subject Not Available
 
Description Not Available
A non-radio-labeled probe-based detection method was developed for rapid enumeration of Salmonella in seafood and water samples. A Salmonella-specific invA gene probe was developed using a digoxigenin-based non-radio labeling assay, which was evaluated with naturally contaminated seafood and water samples. The probe-based technique was further compared with the quantitative PCR assay. The method was specific for detection of different Salmonella serovars without any nonspecific hybridization with other Salmonella-related Enterobacteriaceae. The optimum labeling efficiency was determined for the labeled probe, and 10 pg per μL probe concentration was observed to be most efficient for detection of Salmonella colonies on nylon membrane. Quantification of Salmonella in naturally contaminated seafood and water samples was in the range 10–102 CFU per mL. The assay successfully quantified Salmonella in spiked seafood and water samples in the presence of background flora, and the entire assay was completed within 48 h. The probe-based assay was further evaluated with real-time PCR, and results showed that the assay was comparable to real-time PCR assay. Thus, this probe-based assay can be a rapid, useful, and alternative technique for quantitative detection of Salmonella in food, feed, and water samples.
Not Available
 
Date 2021-07-23T06:50:33Z
2021-07-23T06:50:33Z
2012-11-01
 
Type Research Paper
 
Identifier Rakesh Kumar and Lalitha, K. V. (2012) Digoxigenin labelled probe based colony assay for rapid quantification of Salmonella serovars in seafood and water. J. AOAC Intl. 95(6): 1652-1655.
1060-3271
http://krishi.icar.gov.in/jspui/handle/123456789/49478
 
Language English
 
Relation Not Available;
 
Publisher AOAC International