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Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India

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Title Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India
Not Available
 
Creator Arathi Dharmaratnam
Raj kumar
V. S. Basheer
Neeraj Sood
T. Raja Swaminathan
J. K. Jena
 
Subject Bacterial fish diseases,
Serratia marcescens
Guppy Poecilia reticulata,
 
Description Not Available
Pathogenic strain of Serratia marcescens (NPSM-1) with multiple drug resistance was isolated from guppy Poecilia reticulata with clinical signs of fin rot and was confirmed by biochemical tests and 16S rRNA gene sequencing. The extra cellular proteins (ECP) of the bacteria exhibited marked cytotoxic activity in vitro on Cyprinus carpio koi fin (CCKF) cell line. The in vivo challenge studies confirmed that the isolate was highly pathogenic to fish when the fishes were injected with 1 x 104 CFU/fish and the same bacterium was re-isolated from infected fish, post-challenge. S. marcescens produced large zones of haemolysis on 10% sheep blood agar. The bacteria was found to carry virulence genes; extracellular metallo-protease gene (Pr596) and AHL synthase gene (SpnI). The bacterial isolate was tested to determine sensitivity against 16 antibiotics and was sensitive to only 5 viz., cefixime, chloramphenicol, ciprofloxacin, gentamycin and erythromycin. The study indicates that S. marcescens can cause disease in ornamental fish and the bacterium being a known human pathogen, may also cause infections in humans having direct contact with infected fishes. This is the first report describing S. marcescens as a pathogen of freshwater ornamental fish in India.
Not Available
 
Date 2021-07-24T05:07:59Z
2021-07-24T05:07:59Z
2017-12-01
 
Type Research Paper
 
Identifier Arathi Dharmaratnam, Raj kumar, V. S. Basheer, Neeraj Sood, T. Raja Swaminathan* and J. K. Jena. 2017. Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India. Indian Journal of Fisheries, 64(4): 71-79. DOI: 10.21077/ijf.2017.64.4.71261-10
0970-6011
http://krishi.icar.gov.in/jspui/handle/123456789/49651
 
Language English
 
Relation Not Available;
 
Publisher ICAR - Central Marine Fisheries Research Institute