Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India
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Title |
Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India
Not Available |
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Creator |
Arathi Dharmaratnam
Raj kumar V. S. Basheer Neeraj Sood T. Raja Swaminathan J. K. Jena |
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Subject |
Bacterial fish diseases,
Serratia marcescens Guppy Poecilia reticulata, |
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Description |
Not Available
Pathogenic strain of Serratia marcescens (NPSM-1) with multiple drug resistance was isolated from guppy Poecilia reticulata with clinical signs of fin rot and was confirmed by biochemical tests and 16S rRNA gene sequencing. The extra cellular proteins (ECP) of the bacteria exhibited marked cytotoxic activity in vitro on Cyprinus carpio koi fin (CCKF) cell line. The in vivo challenge studies confirmed that the isolate was highly pathogenic to fish when the fishes were injected with 1 x 104 CFU/fish and the same bacterium was re-isolated from infected fish, post-challenge. S. marcescens produced large zones of haemolysis on 10% sheep blood agar. The bacteria was found to carry virulence genes; extracellular metallo-protease gene (Pr596) and AHL synthase gene (SpnI). The bacterial isolate was tested to determine sensitivity against 16 antibiotics and was sensitive to only 5 viz., cefixime, chloramphenicol, ciprofloxacin, gentamycin and erythromycin. The study indicates that S. marcescens can cause disease in ornamental fish and the bacterium being a known human pathogen, may also cause infections in humans having direct contact with infected fishes. This is the first report describing S. marcescens as a pathogen of freshwater ornamental fish in India. Not Available |
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Date |
2021-07-24T05:07:59Z
2021-07-24T05:07:59Z 2017-12-01 |
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Type |
Research Paper
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Identifier |
Arathi Dharmaratnam, Raj kumar, V. S. Basheer, Neeraj Sood, T. Raja Swaminathan* and J. K. Jena. 2017. Isolation and characterization of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India. Indian Journal of Fisheries, 64(4): 71-79. DOI: 10.21077/ijf.2017.64.4.71261-10
0970-6011 http://krishi.icar.gov.in/jspui/handle/123456789/49651 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ICAR - Central Marine Fisheries Research Institute
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