Record Details

Oxidative Stress and Nano-Toxicity Induced by TiO2 and ZnO on WAG Cell Line

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Oxidative Stress and Nano-Toxicity Induced by TiO2 and ZnO on WAG Cell Line
Not Available
 
Creator Akhilesh Dubey
Mukunda Goswami
Kamalendra Yadav
Dharmendra Chaudhary
 
Subject Oxidative stress
Nano toxicity
WAG cell line
Wallago attu
genotoxicity assays
oxidative stress
In vitro
 
Description Not Available
Metallic nanoparticles are widely used in cosmetics, food products and textile industry. These particles are known to cause respiratory toxicity and epithelial inflammation. They are eventually released to aquatic environment necessitating toxicity studies in cells from respiratory organs of aquatic organisms. Hence, we have developed and characterized a new cell line, WAG, from gill tissue of Wallago attu for toxicity assessment of TiO2 and ZnO nanoparticles. The efficacy of the cell line as an in vitro system for nanoparticles toxicity
studies was established using electron microscopy, cytotoxicity assays, genotoxicity assays and oxidative stress biomarkers. Results obtained with MTT assay, neutral red uptake assay and lactate dehydrogenase assay showed acute toxicity to WAG cells with IC50 values of 25.29±0.12, 34.99±0.09 and 35.06±0.09 mg/l for TiO2 and 5.716±0.1, 3.160±0.1 and 5.57±0.12 mg/l for ZnO treatment respectively. The physicochemical properties and size distribution of nanoparticles were characterized using electron microscopy with integrated
energy dispersive X-ray spectroscopy and Zetasizer. Dose dependent increase in DNA damage, lipid peroxidation and protein carbonylation along with a significant decrease in activity of Superoxide Dismutase, Catalase, total Glutathione levels and total antioxidant capacity with increasing concentration of exposed nanoparticles indicated that the cells were under oxidative stress. The study established WAG cell line as an in vitro system to study toxicity mechanisms of nanoparticles on aquatic organisms.
Department of Biotechnology, India is deeply acknowledged for the financial support. Grant number: BT/PR13538/AAQ/03/506/2010; dated 09.11.2010.
 
Date 2021-07-26T10:49:30Z
2021-07-26T10:49:30Z
2015-05-26
 
Type Research Paper
 
Identifier Dubey A, Goswami M, Yadav K, Chaudhary D (2015) Oxidative Stress and Nano-Toxicity Induced by TiO2 and ZnO on WAG Cell Line. PLoS ONE 10(5): e0127493. https://doi.org/10.1371/journal.pone.0127493
1932-6203
http://krishi.icar.gov.in/jspui/handle/123456789/50198
 
Language English
 
Relation Not Available;
 
Publisher PLOS