Record Details

Establishment and Characterization of Eye Muscle Cell Line from Snow Trout, Schizothorax richardsonii (Gray, 1832), a Vulnerable Coldwater Fish, for In Vitro Studies

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Establishment and Characterization of Eye Muscle Cell Line from Snow Trout, Schizothorax richardsonii (Gray, 1832), a Vulnerable Coldwater Fish, for In Vitro Studies
Not Available
 
Creator Sanjeev Kumar Murali
Pankaj Soni
Neha Singh
Ravindra Kumar
Shreya Srivastava
Akhilesh Kumar Mishra
Vijay Kumar Singh
Basdeo Kushwaha.
 
Subject Cell line
Cytotoxicity
India
Mercury chloride
NRFC
Schizothorax richardsonii
 
Description Not Available
Schizothorax richardsonii, commonly called snow trout is a vulnerable coldwater fish distributed in India and adjoining countries, is an important resource as food, ornamental and game fishery. A cell line, designated as SREM-1, has been established from the eye muscle of S. richardsonii using explant method and cultured in Leibovitz’s L-15 medium. The growth studies at 20-32°C temperatures and 5-20% of fetal bovine serum (FBS) concentrations revealed that SREM-1 cells exhibited optimal growth at 28°C in L-15 medium containing 20% FBS. Cell type was confirmed primarily as epithelial in nature by immuno-phenotyping assay and was sub-cultured for >55 times since its development. The origin of the cell line was confirmed by sequencing of
cytochrome oxidase c subunit I and 16S rRNA genes. SREM-1 cell line is free from mycoplasma contamination and has been cryopreserved at different passage levels with revival efficiency of 70-80% after 6 months. The SREM-1 cell line was successfully transfected and also employed for evaluating in vitro cytotoxicity against a heavy metal, mercuric chloride. The newly established cell line of this vulnerable species would be useful as a model for aquatic toxicological and transfection studies.
Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi
 
Date 2021-07-24T10:35:45Z
2021-07-24T10:35:45Z
2020-12-10
 
Type Research Paper
 
Identifier Murali Sanjeev Kumar, Pankaj Soni, Neha Singh, Ravindra Kumar, Shreya Srivastava, Akhilesh Kumar Mishra, Vijay Kumar Singh and Basdeo Kushwaha. 2021. Establishment and Characterization of Eye Muscle Cell Line from Snow Trout, Schizothorax richardsonii (Gray, 1832), a Vulnerable Coldwater Fish, for In Vitro Studies. Turkish Journal of Fisheries & Aquatic Sciences, 21(2): 95-105.
1303-2712
http://krishi.icar.gov.in/jspui/handle/123456789/49872
 
Language English
 
Relation Not Available;
 
Publisher Central Fisheries Research Institute of Turkey and Japan International Cooperation Agency