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Development of caudal fin cell line from hill trout Barilius bendelisis (Hamilton, 1807) for cytotoxicity and transfection studies

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Title Development of caudal fin cell line from hill trout Barilius bendelisis (Hamilton, 1807) for cytotoxicity and transfection studies
Not Available
 
Creator Murali Sanjeev Kumar
Pankaj Soni
Ravindra Kumar
Neha Singh
Shreya Srivastava
Akhilesh Kumar Mishra
Vijay Kumar Singh
Basdeo Kushwaha.
 
Subject Barilius bendelisis
Cell line
Heavy metal
cytotoxicity
NRFC
 
Description Not Available
A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/ pollutant and gene expression.
Department of Biotechnology, Government of India, New Delhi, India
 
Date 2021-07-28T07:33:42Z
2021-07-28T07:33:42Z
2020-10-14
 
Type Research Paper
 
Identifier Kumar, M. S., Soni, P., Kumar, R., Singh, N., Srivastava, S., Mishra, A. K., Singh, V. K., Kushwaha, B. (2021): Development of caudal fin cell line from hill trout Barilius bendelisis (Hamilton, 1807) for cytotoxicity and transfection studies. Croatian Journal of Fisheries, 79, 15-24. DOI: 10.2478/ cjf-2021-0002.
1330-061X
http://krishi.icar.gov.in/jspui/handle/123456789/50613
 
Language English
 
Relation Not Available;
 
Publisher University of Zagreb Faculty of Agriculture Department of Fisheries, Beekeeping, Game Management and Special Zoology