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Resumption of donor-origin spermatogenesis in senescent goldfish Carassius auratus (Linnaeus, 1758) following spermatogonia cell therapy

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Title Resumption of donor-origin spermatogenesis in senescent goldfish Carassius auratus (Linnaeus, 1758) following spermatogonia cell therapy
Stem cell therapy in fishes
 
Creator Sullip Kumar Majhi
Mog Choudhury
Santosh Kumar
Rajeev Kumar Singh
Vindhya Mohindra
Kuldeep Kumar Lal
 
Subject Senescence
Reproduction
Spermatogenesis
Aquaculture
Goldfish
 
Description Not Available
Stem cell research has come into prominence because of its applications in assisted
reproductive technology and the treatment of deadly diseases. In teleost fishes,
spermatogonial stem cells have been effectively used to produce surrogate gametes
and progeny through germ cell transplantation technique. The present study is the first
report of an innovative application of stem cell therapy in fish species for revitalising the
reproductive competence of senescent individuals. Senescent male goldfish, Carassius
auratus, approximately 10 years of age were procured from a fish-breeding farm and
were reared locally in the lab for an additional two years. The senescence of the
individuals was then evaluated and confirmed using histological analysis, gonadal index
assessment, and germ-cell specific vasa gene expression. Analyses revealed absence of
spermatogonial cells and other germ cells in the testes of the senescent fish (n = 5).
Spermatogonial cells from sexually immature C. auratus male donor were isolated
using discontinuous percoll gradients, labelled with the fluorescent dye PKH-26, and
transplanted into the gonads of senescent C. auratus males through urogenital papilla.
Six months after the transplant, spermatozoa were collected through applying gentle
manual pressure on the abdomen and were observed under a microscope. All C. auratus
males with the transplant had produced spermatozoa from the transplanted cells. This
was confirmed by the retention of PKH-26 in the spermatozoa and diagnostic SSR locus.
Gravid C. auratus females were artificially inseminated with the spermatozoa of those
senescent males and natural spawning was allowed. As a result viable progeny were
produced. These observation suggests that the reproductive competence of senescent
male fishes can be revitalised through spermatogonial stem cell therapy to produce
functional gametes.
This work was supported by a Grant-in-Aid from the National Bureau of Fish Genetic Resources (Institutional research grant # IXX10896).
 
Date 2021-07-24T11:10:26Z
2021-07-24T11:10:26Z
2020-08-13
 
Type Research Paper
 
Identifier Majhi SK, Chowdhury M, Kumar S, Singh RK, Mohindra V, Lal KK. 2020. Resumption of donor-origin spermatogenesis in senescent goldfish Carassius auratus (Linnaeus, 1758) following spermatogonial cell therapy. PeerJ 8:e9116 https://doi.org/10.7717/peerj.9116
2376-5992
http://krishi.icar.gov.in/jspui/handle/123456789/49900
 
Language English
 
Relation Not Available;
 
Publisher PeerJ Inc.