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Isolation and characterization of hypoxia inducible gene connective tissue growth factor (CTGF) in Labeo rohita

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Title Isolation and characterization of hypoxia inducible gene connective tissue growth factor (CTGF) in Labeo rohita
Not Available
 
Creator Iliyas Rashid
Vishwamitra Singh Baisvar
Mahender Singh
Prachi Srivastava
Ravindra Kumar
Basdeo Kushwaha
Ajey Kumar Pathak
 
Subject Database
HIF
HRE
Hypoxia
Promoter
Transcription
 
Description Not Available
The connective tissue growth factor gene plays important role in several biological processes and also responsive to hypoxia stress in fishes. The freshwater fish, Labeo rohita, highly cultured in Indian subcontinent for food, is reported as hypoxia sensitive but annotation and sequences of nuclear genes were not available for this species so far in the public domain, except some transcripts. In this study, an attempt was made for isolation and annotation of the CTGF gene in L. rohita using information of zebrafish from the same family. The CTGF gene sequence was obtained by aligning assembled genome of L. rohita, (NCBI BioProject ID: PRJNA437789), with the CTGF protein of zebrafish. Eight overlapping sets of forward and
reverse primers from aligned region were designed for amplification of around 600 bp long successive overlapping fragments of CTGF gene in L. rohita. Assembly and annotation of overlapping fragments confirmed a complete 2421 bp long CTGF gene sequence with a full coding region that comprised of five exons between 308 and 1921 positions. This annotated CTGF gene sequence was submitted to GenBank (Acc. No. KY940466). Characterization of CTGF will be an initiative in identification of hypoxia response genes in L. rohita which may further help in understanding the mechanism of hypoxia tolerability in this species.
CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi,
 
Date 2021-07-28T07:28:20Z
2021-07-28T07:28:20Z
2019-04-01
 
Type Research Paper
 
Identifier Rashid, I., Baisvar, V.S., Singh, M., Srivastava, P., Kumar, R., Kushwaha, B. and Pathak, A.K., 2019. Isolation and characterization of hypoxia inducible gene connective tissue growth factor (CTGF) in Labeo rohita. Molecular biology reports, 46(2), pp.1683-1691.
ISSN: 0301-4851 (print);
1573-4978 (web)
http://krishi.icar.gov.in/jspui/handle/123456789/50608
 
Language English
 
Relation Not Available;
 
Publisher Springer