Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes
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Title |
Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes
Not Available |
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Creator |
Ravindra Kumar
Vishwamitra Singh Baisvar Basdeo Kushwaha S Murali Vijay Kumar Singh |
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Subject |
BAC clones
BAC DNA BAC end sequencing · Clarias magur · FISH Clarias magur FISH |
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Description |
Not Available
Bacterial artificial chromosome (BAC) library is an important genomic resource useful in targeted marker development, positional cloning, physical mapping and a substrate for genome sequencing for better understanding the genome organization of a species. The present manuscript elucidates the improvement in protocols for economical and efficient BAC insert DNA isolation, BAC end sequencing and FISH for physical localization on the metaphase chromosome complements. BAC clones of Clarias magur, maintained in 384—well plate format in our laboratory, were used in this study. The protocols gave consistent and efficient results. We use routinely these protocols for BAC insert DNA extraction, generating end sequence data of the clone and constructing DNA probes to hybridize on the metaphase spreads of C. magur using FISH for physical their localization. CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi, |
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Date |
2021-07-28T10:19:23Z
2021-07-28T10:19:23Z 2020-05-02 |
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Type |
Research Paper
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Identifier |
Kumar, R., Baisvar, V.S., Kushwaha, B., Murali, S. and Singh, V.K., 2020. Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes. Molecular biology reports, 47(3), pp.2405-2413.
1573-4978 (web) 0301-4851 (print) http://krishi.icar.gov.in/jspui/handle/123456789/50777 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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