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Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita

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Title Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita
Not Available
 
Creator Iliyas Rashid
Vishwamitra Singh Baisvar
Mahender Singh
Ravindra Kumar
Prachi Srivastava
Basdeo Kushwaha
Ajey Kumar Pathak
 
Subject HMOX1
Hypoxia
Labeo rohita
Phylogenetics
SSR
 
Description Not Available
The HMOX1 gene plays role in several biological processes and is also responsive to hypoxia stress. Freshwater carp fish, Labeo rohita, is reported as hypoxia sensitive, but the information of annotated hypoxia genes in public domain is very scanty for this species. Here, an attempt was made to isolate and characterize HMOX1 gene in L. rohita using information from zebrafish. HMOX1 gene was obtained by mapping HMOX1 protein of zebrafish over assembled genome of L. rohita. Aligned region was used for designing primers for HMOX1 amplification. Eight overlapping sets of primers were designed for amplifying ~540 bp long successive overlapping fragments. Splicing of overlapping amplicons generated 3715 bp fragment that was confirmed as HMOX1 gene having full coding region with 6 exons between 184 and 2156 bp positions. HMOX1 characterization is an initiative for L. rohita genes annotation to support the characterization of new genes in the important species.
CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi,
 
Date 2021-07-28T10:26:09Z
2021-07-28T10:26:09Z
2020-05-01
 
Type Research Paper
 
Identifier Rashid, I., Baisvar, V.S., Singh, M., Kumar, R., Srivastava, P., Kushwaha, B. and Pathak, A.K., 2020. Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita. Genomics, 112(3), pp.2327-2333.
0888-7543 (print)
1089-8646 (web)
http://krishi.icar.gov.in/jspui/handle/123456789/50783
 
Language English
 
Relation Not Available;
 
Publisher Academic Press