Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita
Not Available |
|
Creator |
Iliyas Rashid
Vishwamitra Singh Baisvar Mahender Singh Ravindra Kumar Prachi Srivastava Basdeo Kushwaha Ajey Kumar Pathak |
|
Subject |
HMOX1
Hypoxia Labeo rohita Phylogenetics SSR |
|
Description |
Not Available
The HMOX1 gene plays role in several biological processes and is also responsive to hypoxia stress. Freshwater carp fish, Labeo rohita, is reported as hypoxia sensitive, but the information of annotated hypoxia genes in public domain is very scanty for this species. Here, an attempt was made to isolate and characterize HMOX1 gene in L. rohita using information from zebrafish. HMOX1 gene was obtained by mapping HMOX1 protein of zebrafish over assembled genome of L. rohita. Aligned region was used for designing primers for HMOX1 amplification. Eight overlapping sets of primers were designed for amplifying ~540 bp long successive overlapping fragments. Splicing of overlapping amplicons generated 3715 bp fragment that was confirmed as HMOX1 gene having full coding region with 6 exons between 184 and 2156 bp positions. HMOX1 characterization is an initiative for L. rohita genes annotation to support the characterization of new genes in the important species. CABin Scheme of ICAR and CABin Division of ICAR-IASRI, New Delhi, |
|
Date |
2021-07-28T10:26:09Z
2021-07-28T10:26:09Z 2020-05-01 |
|
Type |
Research Paper
|
|
Identifier |
Rashid, I., Baisvar, V.S., Singh, M., Kumar, R., Srivastava, P., Kushwaha, B. and Pathak, A.K., 2020. Isolation and characterization of hypoxia inducible heme oxygenase 1 (HMOX1) gene in Labeo rohita. Genomics, 112(3), pp.2327-2333.
0888-7543 (print) 1089-8646 (web) http://krishi.icar.gov.in/jspui/handle/123456789/50783 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Academic Press
|
|