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Chromosome analysis of walking catfish, Clarias magur (Teleostei, Siluriformes, Clariidae), using staining, FISH with 18S, 5S and BAC probes

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Title Chromosome analysis of walking catfish, Clarias magur (Teleostei, Siluriformes, Clariidae), using staining, FISH with 18S, 5S and BAC probes
Not Available
 
Creator Vishwamitra Singh Baisvar
Basdeo Kushwaha
Ravindra Kumar
Murali Sanjeev Kumar
Manmohan Pandey
Mahender Singh
 
Subject BAC clone
Chromosome
FISH
Karyotype
 
Description Not Available
The chromosomal characteristics of Clarias magur were examined using conventional (Giemsa-staining, Ag-impregnation and CMA3 + DAPI fluorescence) and molecular/ FISH (18S & 5S rDNA probes + one BAC DNA probe) cytogenetic tools. The diploid chromosome number was 50 and the karyotype consisted of 14 metacentric, 20 sub-metacentric, 8 sub-telocentric, 8 acrocentric chromosomes with 84 chromosome arms without any heteromorphic pair. The C-heterochromatic blocks were located on centromeric position of 13 pairs of chromosomes. The NOR sites, visualized by AgNO3-and CMA3-staining, were situated at p arms of chromosome pair No. 21, which also corresponded to 18S rDNA site visualized by FISH. The FISH signal of
ICF_001_D19 clone probe was observed on 18th chromosome pair. The findings of the present study on C. magur provided valuable markers for the chromosome identification and locations of genes of the BAC clone on the chromosome will lead to the construction of physical map of genome of this species.
CABin Scheme of ICAR, implemented through ICAR-Indian Agricultural Statistics Research Institute (IASRI), New Delhi,
 
Date 2021-07-28T10:42:55Z
2021-07-28T10:42:55Z
2021-02-24
 
Type Research Paper
 
Identifier Singh Baisvar, V., Kushwaha, B., Kumar, R., Sanjeev Kumar, M., Pandey, M. and Singh, M., 2021. Chromosome analysis of walking catfish, Clarias magur (Teleostei, Siluriformes, Clariidae), using staining, FISH with 18S, 5S and BAC probes. Journal of Applied Ichthyology.
0175-8659 (print)
1439-0426 (web)
http://krishi.icar.gov.in/jspui/handle/123456789/50799
 
Language English
 
Relation Not Available;
 
Publisher Wiley