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Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications

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Title Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications
Not Available
 
Creator Neha Singh
Pankaj Soni
Basdeo Kushwaha
Murali Sanjeev Kumar
J. K. Srivastava
Shreya Srivastava
Akhilesh Kumar Mishra
Ravindra Kumar
 
Subject Cell line
Clarias magur
Cytotoxicity
Heavy metals
Testis
 
Description Not Available
We developed a gonad cell line, CMgT-1, from the testes of Clarias magur in L-15 media with 20% FBS at 28 °C temperature and sub-cultured it more than 50 times. The origin of the cell line was validated using two universal markers, viz. cytochrome oxidase c subunit I and 16S rRNA genes. Cytogenetic analysis of the cells at 32nd passage revealed diploid chromosome number of 50. Immuno-phenotyping assay confirmed an epithelial-like cell type. The cell line was tested for mycoplasma contamination and found to be negative. The cells were successfully transfected with a GFP reporter gene that indicated that the cell line could be utilized in gene expression studies. The cell line was successfully utilized for evaluating cytotoxic effects of heavy metals, viz. potassium dichromate, mercury chloride and sodium (meta) arsenite, which reflects its potential
applications in toxicity studies. CMgT-1 cell line will serve as a valuable resource for cytotoxicity and other in vitro studies.
Department of Biotechnology, Government of India, New Delhi, India, vide Sanction Order No. BT/PR13908/ AAQ/3/733/2015
 
Date 2021-07-29T05:51:33Z
2021-07-29T05:51:33Z
2021-01-01
 
Type Research Paper
 
Identifier Singh, N., Soni, P., Kushwaha, B., Kumar, M.S., Srivastava, J.K., Srivastava, S., Mishra, A.K. and Kumar, R., 2021. Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications. The Nucleus,64:211–217
0029-568X (Print )
0976-7975 (Electronic)
http://krishi.icar.gov.in/jspui/handle/123456789/50988
 
Language English
 
Relation Not Available;
 
Publisher Springer India