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Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research.

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Title Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research.
Not Available
 
Creator Naresh Sahebrao Nagpure
Akhilesh Kr. Mishra
A. S. Ninawe
Avinash Rasal
Akhilesh Dubey
Amit Kumar
M. Goswami
Ravindra Kumar
J. K. Jena
 
Subject Cell line repository
Cryopreservation
Fish cell line
Molecular characterization
 
Description Not Available
Fish cell line has emerged as an important tool in fishery biotechnology. In recent years, various fish cell lines have been developed by different researchers across the country. National Repository on Fish cell lines, established with the aim to preserve fish cell lines for training and education to stakeholders, has started functioning at National Bureau of Fish Genetic Resources, Lucknow. This repository is supposed to characterize and preserve the fish cell lines developed across the country and serve as a national referral centre for Indian and exotic fish cell lines. Currently, the repository is maintaining 50 fish cell lines deposited by various research institutes in India, including the cell lines developed at cell culture facility of National Bureau of Fish Genetic Resources. The cell lines have been successfully cryopreserved after verifying its authenticity by sequence analysis of two mitochondrial genes, viz. 16S rRNA and cytochrome c oxidase sub-unit I. Chromosomal analysis, transfection efficiency and immunocytochemistry are also being used to characterize the cell lines. The facility is serviceable for the collection, deposition and distribution of fish cell lines. This paper discusses the status as well as the methodology adopted for fish cell lines development, characterization and storage at NRFC.
Department of Biotechnology, Government of India, New Delhi.
 
Date 2021-07-28T07:23:05Z
2021-07-28T07:23:05Z
2015-12-22
 
Type Research Paper
 
Identifier Nagpure, N.S., Mishra, A.K., Ninawe, A.S. et al. Molecular and Cytogenetic Characterization of Fish Cell Lines and its Application in Aquatic Research. Natl. Acad. Sci. Lett. 39, 11–16.
2250-1754
0250-541X
http://krishi.icar.gov.in/jspui/handle/123456789/50605
 
Language English
 
Relation Not Available;
 
Publisher Springer