Detection of Virulence Genes in Aeromonas sobria Isolated from Diseased Fish Rohu, Labeo rohita by PCR
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Title |
Detection of Virulence Genes in Aeromonas sobria Isolated from Diseased Fish Rohu, Labeo rohita by PCR
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Creator |
Chandra Bhushan Kumar
Shilpa kumari Anil Kumar Shivanand Chinmayee Muduli Vikash Sahu S. M. Srivastava Gaurav Rathore |
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Subject |
Aeromonas sobria
Virulence genes Cytotoxic enterotoxin Extracellular hemolysin Enolase Cytotonic enterotoxin |
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Description |
The Genus Aeromonas belongs to facultative, anaerobic gram negative bacteria. Several mesophilic species of Aeromonas cause septicaemia in a variety of aquatic organisms and gastrointestinal diseases in humans. The virulence of Aeromonas is considered multifactorial and are known to possess aerolysins, hemolysins, proteases, phospholipases, lipases, adhesions and enterotoxins. Therefore, the importance of present work lies in detecting the virulence genes in Aeromonas sobria isolated from diseased fish, rohu. The bacterium was identified using VITEK 2, automated bacterial identification system. Out of four genes screened, three genes namely, actA (cytotoxic enterotoxin), ahh1A (extracellular hemolysin) and enoA (enolase) were detected in A. sobria by PCR. However, astA gene which encodes for heat-stable cytotonic enterotoxin was not detected.
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Date |
2021-07-29T07:00:16Z
2021-07-29T07:00:16Z 2020-01-20 |
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Type |
Research Paper
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Identifier |
Chandra Bhushan Kumar, Shilpa kumari, Anil Kumar, Shivanand, Chinmayee Muduli, Vikash Sahu, S. M. Srivastava and Gaurav Rathore. 2020. Detection of Virulence Genes in Aeromonas sobria Isolated from Diseased Fish Rohu, Labeo rohita by PCR.Int.J.Curr.Microbiol.App.Sci. 9(1): 884-890. doi: https://doi.org/10.20546/ijcmas.2020.901.098
2319-7706 (Online) 2319-7692 (Print) http://krishi.icar.gov.in/jspui/handle/123456789/51004 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Excellent publishers
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