Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840
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Title |
Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840
Not Available |
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Creator |
T. Raja Swaminathan
Arathi Dharmaratnam S. Arun Raja N. Sundar Raj Kuldeep K Lal |
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Subject |
Clarias dussumieri,
cryopreservation cell line |
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Description |
Engaged in all aspects of fish biology
We describe a new cell line, Clarias dussumieri fin (ClDuF), from the caudal fin of C. dussumieri using the explant technique followed by cryopreservation. The cryopreserved CiDuF cells were validated for quality and other characteristics. They showed typical epithelial morphology in vitro and epithelial cells outgrew their fibroblast cells after the fifth passage. ClDuF cells had a characteristic sigmoid curve with population doubling in 24 h. Immunotyping of the ClDuF cells against cytokeratin suggested the epithelial lineage. Chromosome analysis showed normal diploid (2n = 50) numbers and the cells did not contain any contamination, including Mycoplasma and other microbes. Partial sequencing of fragments of mitochondrial 16s rRNA and COI genes of ClDuF confirmed that the cell line was initiated from C. dussumieri. Cells at the 10th and 25th passages had more than 80% and 70% viability in the culture, respectively, after 6 months of storage at LN2 . These ClDuF cells were morphologically identical to the cells before freezing and the genetic resource of C. dussumieri was preserved. The species-specific cells can serve as a valuable source for virus isolation, conservation and cloning of somatic cells. Director, ICAR-National Bureau of Fish Genetic Resources, Lucknow, India, and also to the Director General, Indian Council of Agricultural Research, New Delhi and Secretary Department of Agriculture, Department of Agriculture Education and Reserach, Government of India, New Delhi, and Deputy Director General (Fy. Sc.), Indian Council of Agricultural Research, New Delhi for funding support, guidance and encouragement. |
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Date |
2021-08-03T07:35:50Z
2021-08-03T07:35:50Z 2020-01-27 |
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Type |
Research Paper
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Identifier |
Swaminathan, T. R., Arathi Dharmaratnam, S. Arun Raja, Sundar Raj, and Kuldeep Kumar Lal. 2020. Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840. Journal: Journal of Fish Biology, DOI: 10.1111/jfb.14265.
1095-8649 http://krishi.icar.gov.in/jspui/handle/123456789/52525 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Wiley
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