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DNA barcoding Indian freshwater fishes

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Title DNA barcoding Indian freshwater fishes
Not Available
 
Creator Wazir Singh Lakra
M Singh
Mukunda Goswami
A Gopalakrishnan
K K Lal
V Mohindra
U K Sarkar
P P Punia
K V Singh
J P Bhatt
S Ayyappan
 
Subject DNA barcode
COI
Genetic divergence
Phylogeny
 
Description Not Available
DNA barcoding is a promising technique for species identification using a short mitochondrial DNA sequence of cytochrome c oxidase I (COI) gene. In the present study, DNA barcodes were generated from 72 species of freshwater fish covering the Orders Cypriniformes, Siluriformes, Perciformes, Synbranchiformes, and Osteoglossiformes representing 50 genera and 19 families. All the samples were collected from diverse sites except the species endemic to a particular location. Species were represented by multiple specimens in the great majority of the barcoded species. A total of 284 COI sequences were generated. After amplification and sequencing of 700 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.40%, 9.60%, 13.10%, and 17.16%, respectively. DNA barcode discriminated congeneric species without any confusion. The study strongly validated the efficiency of COI as an ideal marker for DNA barcoding of Indian freshwater fishes.
Not Available
 
Date 2021-08-03T08:31:37Z
2021-08-03T08:31:37Z
2015-12-24
 
Type Research Paper
 
Identifier Lakra, W.S., Singh, M., Goswami, M., Gopalakrishnan, A., Lal, K.K., Mohindra, V., Sarkar, U.K., Punia, P.P., Singh, K.V., Bhatt, J.P. and Ayyappan, S. (2016) DNA barcoding Indian freshwater fishes, Mitochondrial DNA Part A, 27:6, 4510-4517, DOI: 10.3109/19401736.2015.1101540
2470-1394
2470-1408
http://krishi.icar.gov.in/jspui/handle/123456789/52574
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis