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A simple method of DNA isolation from jute (Corchorus olitorius) seed suitable for PCR-based detection of the pathogen Macrophomina phaseolina (Tassi) Goid.

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Title A simple method of DNA isolation from jute (Corchorus olitorius) seed suitable for PCR-based detection of the pathogen Macrophomina phaseolina (Tassi) Goid.
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Creator C. Biswas , P. Dey , S. Satpathy , S.K. Sarkar , A. Bera and B.S. Mahapatra
 
Subject DNA isolation, jute seed, Macrophomina phaseolina, PCR.
 
Description Not Available
A simple method was developed for isolating DNA from jute seed, which
contains high amounts of mucilage and secondary metabolites, and a PCR
protocol was standardized for detecting the seedborne pathogen Macrophomina
phaseolina. The cetyl trimethyl ammonium bromide method was modified with
increased salt concentration and a simple sodium acetate treatment to extract
genomic as well as fungal DNA directly from infected jute seed. The Miniprep
was evaluated along with five other methods of DNA isolation in terms of
yield and quality of DNA and number of PCR positive samples. The Miniprep
consistently recovered high amounts of DNA with good spectral qualities at
A260/A280. The DNA isolated from jute seed was found suitable for PCR
amplification. Macrophomina phaseolina could be detected by PCR from
artificially inoculated as well as naturally infected jute seeds. The limit of PCRbased detection of M. phaseolina in jute seed was determined to be
0 62 9 107 CFU g1 seed.
Not Available
 
Date 2020-08-04T05:24:03Z
2020-08-04T05:24:03Z
2012-11-05
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38914
 
Language English
 
Relation Not Available;
 
Publisher Wiley-Blackwell Publishing Ltd