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A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L.)

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Title A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L.)
Not Available
 
Creator C. Biswas, P. Dey and S. Satpathy
 
Subject begomovirus, jute, multiplex nested PCR, phytoplasma.
 
Description Not Available
A multiplex nested PCR assay was developed by optimizing reaction
components and reaction cycling parameters for simultaneous detection of
Corchorus golden mosaic virus (CoGMV) and a phytoplasma (Group 16Sr V-C)
causing little leaf and bunchy top in white jute (Corchorus capsularis). Three
sets of specific primers viz. a CoGMV specific (DNA-A region) primer, a 16S
rDNA universal primer pair P1/P7 and nested primer pair R16F2n/R2 for
phytoplasmas were used. The concentrations of the PCR components such as
primers, MgCl2, Taq DNA polymerase, dNTPs and PCR conditions including
annealing temperature and amplification cycles were examined and optimized.
Expected fragments of 1 kb (CoGMV), 674 bp (phytoplasma) and 370 bp
(nested R16F2n/R2) were successfully amplified by this multiplex nested PCR
system ensuring simultaneous, sensitive and specific detection of the
phytoplasma and the virus. The multiplex nested PCR provides a sensitive,
rapid and low-cost method for simultaneous detection of jute little leaf
phytoplasma and CoGMV. Based on BLASTn analyses, the phytoplasma was
found to belong to the Group 16Sr V-C.
Not Available
 
Date 2020-08-04T05:25:11Z
2020-08-04T05:25:11Z
2012-02-10
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38917
 
Language English
 
Relation Not Available;
 
Publisher Wiley-Blackwell Publishing Ltd