Record Details


Field	Value

Title	A method of direct PCR without DNA extraction for rapid detection of begomoviruses infecting jute and mesta Not Available

Creator	C. Biswas, P. Dey and S. Satpathy

Subject	direct PCR, jute, lysis buffer, mesta.

Description	Not Available We have developed a simple method of direct PCR (dPCR) without timeconsuming procedures of DNA extraction by directly using the leaf bits for rapid detection of begomoviruses in jute and mesta. The leaf bits were treated with a lysis buffer for 35 min, and the lysate was used as PCR template. Different components and their concentration in lysis buffer systems were optimized and the optimal buffer system composed of 20 mmol l 1 tris (hydroxymethyl aminomethane (Tris)-Cl (pH 80)), 15 mmol l 1 ethylenediaminetetraacetic acid (EDTA) (pH 80), 14 mol l 1 NaCl and 200 lg/mL Proteinase K. Further, 3% PVP (w/v) and b-marcaptoethanol (1% v/v) were additionally added into the buffer in case of jute. Under optimized PCR conditions, both viral DNA as well as plant (jute and mesta) genomic DNA were amplified from the lysate. dPCR required fewer reagents and less incubation time reducing both time and cost of detection. Not Available

Date	2020-08-06T08:38:12Z 2020-08-06T08:38:12Z 2013-11-20

Type	Research Paper

Identifier	Not Available Not Available http://krishi.icar.gov.in/jspui/handle/123456789/38999

Language	English

Relation	Not Available;

Publisher	Wiley-Blackwell Publishing Ltd

ICAR Research Data Repository for Knowledge Management