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Molecular identification of a Candidatus phytoplasma (Group16SrV-D) coding partial uvrB gene and degV gene on a new host – mesta (Hibiscus sabdariffa) – with phyllody and reddening of leaves in India

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Title Molecular identification of a Candidatus phytoplasma (Group16SrV-D) coding partial uvrB gene and degV gene on a new host – mesta (Hibiscus sabdariffa) – with phyllody and reddening of leaves in India
Not Available
 
Creator Chinmay Biswas , Piyali Dey , Subrata Satpathy ,Manoj Kumar ,Pratik Satya & B. S. Mahapatra
 
Subject Nested PCR . Roselle . 16SrV. Sequence analysis
 
Description Not Available
Mesta (Hibiscus sabdariffa) is an important
bast fiber crop. In August 2011, there was an outbreak of
a phytoplasma-like disease on H. sabdariffa in different
villages of the northern coastal mesta-growing region of
Andhra Pradesh, India, covering mainly two districts –
Srikakulam and Vijayanagaram. The infected plants
showed characteristic symptoms such as phyllody and
reddening of leaves. PCR with P1/P7 universal primer
pair of 16 S rDNA yielded amplicons of 1850 bp from all
symptomatic mesta leaf samples similar to samples of
brinjal little leaf (phytoplasma positive reference control).
However, asymptomatic samples were not amplified.
Multiplex nested-PCR showed simultaneous amplification of DNA fragments with phytoplasma specific
primers, viz., P1/P7 universal primer pair of 16 S
rDNA, nested primer pair R16F2n/R2, uvrB and DegV
gene-specific uvrB-degVF/R primer generating
amplicons of 1850 bp, 1200 bp and 1023bp, respectively.
However, SecY-map gene specific primer SecY-mapF/R
was not amplified. The 1023 bp nucleotide sequence of
uvrB and DegV gene of the phytoplasma was deposited
in the GenBank (NCBI) with the accession no.
JX975061. NCBI BLASTn analysis of the 1023 bp
products showed that the phytoplasma strain belonged
to elm yellows group (16SrV-D). This is the first report
that Hibiscus sabdariffa is infected by a phytoplasma
and we named it mesta phyllody disease (MPD).
Not Available
 
Date 2020-08-04T05:27:55Z
2020-08-04T05:27:55Z
2013-06-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38918
 
Language English
 
Relation Not Available;
 
Publisher Springer