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Next-generation sequencing and micro RNAs analysis reveal SA/MeJA1/ABA pathway genes mediated systemic acquired resistance (SAR) and its master regulation via production of phased, trans-acting siRNAs against stem rot pathogen Macrophomina phaseolina in a RIL population of jute (Corchorus capsularis)

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Title Next-generation sequencing and micro RNAs analysis reveal SA/MeJA1/ABA pathway genes mediated systemic acquired resistance (SAR) and its master regulation via production of phased, trans-acting siRNAs against stem rot pathogen Macrophomina phaseolina in a RIL population of jute (Corchorus capsularis)
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Creator Chinmay Biswas* , Piyali Dey, P.G. Karmakar, Subrata Satpathy
 
Subject Transcriptome analysis, Next-generation sequencing, Jute RIL, SAR, miRNAs
 
Description Not Available
A RIL population of jute was developed by crossing one resistant accession CIM 036 and a susceptible
variety JRC 412. Two cDNA libraries were constructed using pool of mRNA from healthy as well as
infected seedlings from all the 177 RIL lines. A significant number of defense genes involved in the
defense-response were identified viz. cell wall biosynthesis, reactive oxygen species (ROS), salicylic acid
(SA), ethylene, jasmonic acid (JA), abscissic acid (ABA), hormone signaling, hypersensitive response (HR)
and programmed cell death (PCD) pathways. Furthermore, microRNA analysis revealed that Trans-acting
siRNAs (tasiRNAs) negatively regulate these target transcripts and are characterized by siRNAs spaced in
21-nucleotide (nt) “phased” intervals. We identified highly abundant 22-nt miRNA families that target
conserved domains in these SA/JA/ABA precursors and trigger the production of trans-acting siRNAs. SA
and JA1 transcripts were found to be cleaved by these 22-nt miRNA generating phasiRNA, suggesting
silencing pathogenicity pathway of Macrophomina phaseolina. Gene function annotation was studied in
jute-M. phaseolina interaction in each of the 177 lines of a RIL population. tasiRNA based SAR regulation
demonstrated master regulator of a large gene family. It is the first report of studying resistance
mechanism in jute against M. phaseolina in a RIL population through transcript and miRNA analysis
Not Available
 
Date 2020-08-04T05:30:13Z
2020-08-04T05:30:13Z
2014-07-24
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38919
 
Language English
 
Relation Not Available;
 
Publisher Elsevier