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Discovery of large-scale SNP markers and construction of linkage map in a RIL population of jute (Corchorus capsularis)

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Title Discovery of large-scale SNP markers and construction of linkage map in a RIL population of jute (Corchorus capsularis)
Not Available
 
Creator Chinmay Biswas , Piyali Dey , P. G. Karmakar , Subrata Satpathy
 
Subject Single nucleotide polymorphism Expressed sequence tag Linkage map Jute RIL
 
Description Not Available
Jute is the most important bast fibre crop
of the world. Stem rot disease caused by Macrophomina phaseolina is a major constraint of jute
cultivation. Recent advances in genomics and bioinformatics have enabled the development of highthroughput genotyping, allowing the construction of
saturated linkage maps for identification of resistant
lines. In the present study, single nucleotide polymorphism (SNP) markers associated with expressed
sequence tags (ESTs) were developed and were used
to generate comprehensive linkage maps for Corchorus capsularis. From a set of 43,335 variant nucleotide
positions detected through in silico analysis, 768 SNPs
were selected for genotyping of a recombinant inbred
line population. A total of 705 SNPs (91.7 %)
successfully detected segregating polymorphisms. In
addition to EST–SNPs, genomic simple sequence
repeats were assigned to the genetic map in order to
obtain an evenly distributed genome-wide coverage.
Nine linkage groups were constructed from genetic
maps with estimated cumulative total map length of
2016 cM and average inter-locus interval of 4.2 cM.
SNP assay and linkage mapping are important set of
tools for marker-assisted selection, and it could be
used for identifying resistance to M. phaseolina in jute
cultivars. This is the first report of developing SNP
markers and SNP-based linkage map in jute.
Not Available
 
Date 2020-08-06T05:43:09Z
2020-08-06T05:43:09Z
2015-04-23
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38971
 
Language English
 
Relation Not Available;
 
Publisher Springer