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First Report of Bacterial Leaf Spot Caused by Xanthomonas campestris pv. olitorii on Jute Grown for Seed in India

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Title First Report of Bacterial Leaf Spot Caused by Xanthomonas campestris pv. olitorii on Jute Grown for Seed in India
Not Available
 
Creator C. Biswas, P. Dey, A. Bera, S. Satpathy, and B. S. Mahapatra,
 
Subject Xanthomonas campestris, leaf spot, jute
 
Description Not Available
Jute (Corchorus olitorius L.) is the second most important fiber crop after cotton in terms of
global production (3). In November 2011, symptoms suggestive of bacterial infection were
observed on a seed crop of jute at the CRIJAF research farm, Barrackpore, West Bengal,
India. The disease appeared as small, brown, circular spots, usually less than 5 mm in
diameter on the leaves and some of the spots were surrounded by a yellow halo. The
lesions on the stems were elongated and in some cases were found to girdle the stem. In
the later stages of disease, brown sunken spots were found on the green capsules. Disease
incidence varied from about 20% to 90% of the total plants in different affected fields at
the CRIJAF research farm. Bacterial leaf spot of jute with similar symptoms was reported in
1957 from Sudan (4). Five symptomatic and three asymptomatic leaf samples were
collected from different jute fields. Bacterial colonies isolated on nutrient agar medium from
infected young leaves were Xanthomonas-like and pale yellow cream in color. Total DNA
was extracted from symptomatic as well as asymptomatic leaf samples by using an
improved salt concentration and simple sodium acetate CTAB method (2). Single bacterial
colonies were transferred to nutrient agar (NA) medium plates and incubated at 28°C for 48
h. Pure colonies from plates were used directly for DNA extraction using the QIAGEN
DNeasy Blood and Tissue kit. PCR was carried out with Xanthomonas campestris specific
primers NZ8F3/NZ85R3 (1), which generated an amplicon of 530 bp from all the
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Plant Disease Journal - 97(8):1109 - Abstract
file:///C|/Drive%20E/Published%20Papers/Plant%20Disease%20bacterial%20leaf%20spot.htm[14 - 06 - 2018 12:12:43]
symptomatic leaf samples as well as pure cultures of the isolated bacteria. No amplification
was obtained from asymptomatic leaves. The amplicons from the five symptomatic samples
collected from the field were sequenced and showed 100% identity with one another, and
one sequence (strain JB-CO-13) was deposited in GenBank (Accession No. KC342185). The
BLASTn analysis revealed that bacterial strain JB-CO-13 had 100% identity with X.
campestris pv. olitorii (EU285213). Nucleotide span and ORF finder (NCBI) analysis indicated
the 530-bp PCR amplicon coded part of a gyrase B gene that had 100% identity with a
translated gene product (Protein ID: ABX84334). Three leaves of five 1-month-old jute
plants (cv. JRO 204) in pot culture were infiltrated each with a separate bacterial strain
using suspensions (1 × 105 CFU/ml) in distilled water. The negative control consisted of
leaves infiltrated with sterile distilled water. The plants were kept in a greenhouse with
mean maximum and minimum temperatures of 28.96 and 21.8°C, respectively. The plants
were covered with plastic bags to maintain high relative humidity (>80%). Typical bacterial
lesions were recorded on all the inoculated plants after 1 week. No lesions were seen on the
negative control. To the best of our knowledge, this is the first report of bacterial leaf spot
on C. olitorius caused by X. campestris pv. olitorii from India.
Not Available
 
Date 2020-08-06T08:40:05Z
2020-08-06T08:40:05Z
2013-08-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/39001
 
Language English
 
Relation Not Available;
 
Publisher American Phytopathological Society