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Marker-assisted introgression of genes conferring resistance against bacterial blight & blast into RPHR-1005, the elite restorer line of the popular rice hybrid, DRRH-3

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Title Marker-assisted introgression of genes conferring resistance against bacterial blight & blast into RPHR-1005, the elite restorer line of the popular rice hybrid, DRRH-3
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Creator 80. Abhilash Kumar V, Balachiranjeevi CH, Bhaskar Naik S, Rambabu R, Rekha G, Pranathi K, Hajira SK, Anila M, Mahadevaswamy HK, Harika G, Hariprasad AS, Madhav MS, Laha GS, Prasad MS and Sundaram RM
 
Subject Bacterial blight resistance (Xa21)
Blast resistance (Pi2)
Marker-assisted backcross breeding
RPHR-1005
Fertility restoration
Rf4; Rf3
 
Description Not Available
RPHR-1005 is a stable, elite restorer line of rice possessing highly desirable medium-slender grain type and is the male parent of the popular public bred hybrid DRRH-3. However, both RPHR-1005 and DRRH-3 are highly susceptible to major diseases like bacterial blight (BB) and blast. As genes conferring effective resistance are available against both BB and blast along with availability of gene-specific markers, the present study was carried out to introgress a major BB resistance gene, Xa21 and a major blast resistance gene, Pi2 into the genetic background of RPHR-1005 through marker-assisted backcross breeding. RPBio Patho-1, a breeding line in the genetic background of the popular variety, Samba Mahsuri and possessing Xa21 and Pi2 served as the donor. Marker-assisted backcross breeding strategy was deployed for targeted introgression of the two resistance genes into RPHR-1005. This involved two rounds of backcrossing and at each backcross generation, foreground selection was carried out using PCR based molecular markers specific for Xa21 (i.e. pTA248) and Pi2 (i.e. AP5659-5) along with the markers specific for the major fertility restorer genes, Rf3 (i.e. DRRM-RF3-10) and Rf4 (i.e. DRCG-RF4-14) and background selection was done using a set of 61 parental polymorphic SSR markers spread across the rice genome. At BC2F2, a single plant possessing all the targeted genes along with maximum recurrent parent genome recovery (~ 93.4%; plant # RP-9-27-79-179) was selected and advanced through selfing and pedigree-based selection for morphological traits. AT BC2F4, three lines, viz., RP-9-27-79-179-74-9, RP-9-27-79-179-74-79 and RP-9-27-79-179-74-105, possessing high level of resistance against BB and blast along with complete fertility restoration and all the elite features of RPHR-1005 were identified.
Department of Biotechnology, Government of India File no. BT/PR11705/AGR/02/646/2008.
 
Date 2021-08-05T06:08:14Z
2021-08-05T06:08:14Z
2015-11
 
Type Article
 
Identifier Abhilash Kumar V, Balachiranjeevi CH, Bhaskar Naik S et al. 2015. Marker-assisted introgression of genes conferring resistance against bacterial blight & blast into RPHR-1005, the elite restorer line of the popular rice hybrid, DRRH-3.International Journal of Current Research 7 (11): 22222-22228
0975-833X
http://krishi.icar.gov.in/jspui/handle/123456789/53205
 
Language English
 
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Publisher Not Available