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Expression profiling of immature florets of IR58025A, a wild‑abortive cytoplasmic male sterile line of rice and its cognate, isonuclear maintainer line, IR58025B

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Title Expression profiling of immature florets of IR58025A, a wild‑abortive cytoplasmic male sterile line of rice and its cognate, isonuclear maintainer line, IR58025B
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Creator Pranathi K*, Barbadikar MB*, Viraktamath BC*, Balachandran SM, Hajira SK, Rao PK, Kulkarni SR, Rekha G, Anila M, Kousik MBVN, Senguttuvel P, Hariprasad AS, Mangrautia, Madhav MS and Sundaram RM
 
Subject WA-CMS
Maintainer
IR58025A
IR58025B
RNA-Seq
Hybrid rice
 
Description Not Available
Interaction between gene products encoded by the cytoplasm and nucleus form the core of wild abortive cytoplasmic male sterile (WA-CMS) system of hybrid breeding in rice. Gaining insights into such interactions can be helpful in the development of better three-line rice hybrids and also identify novel male sterility systems. In the present study, the whole transcriptome profiles of immature florets of IR58025A, a WA-CMS line and its isonuclear maintainer line, IR58025B, collected at pre-anthesis stage were compared to delineate the pathways involved in pollen abortion and male sterility. Among the 774 differentially expressed transcripts (DETs), 496 were down regulated and 278 were up regulated in IR58025A compared to IR58025B. The genes associated with oxidative stress response, defense response, etc. were significantly up-regulated, while those associated with respiration, cell wall modifications, pectinesterase activity, etc. were significantly down-regulated in the WA-CMS line. Gene ontology and pathway enrichment analyses revealed the down-regulation of both nuclear and organellar genes involved in key metabolic processes of cell respiration, photosynthesis and other energy yielding metabolites in IR58025A, relative to IR58025B, indicating a general shift toward conservation of energy and other key resources in the florets of WA-CMS line. The data derived from RNA-Seq analysis were validated through qRT-PCR analysis. Based on the results obtained, it can be hypothesized that pollen abortion principally occurs due to up-regulation of pathways leading to oxidative stress leading to energy starvation conditions in consonance with reduced expression of genes associated with the cell wall formation, respiration, and other key metabolic processes.
Department of Biotechnology, Government of India
 
Date 2021-08-03T08:27:33Z
2021-08-03T08:27:33Z
2019-06-21
 
Type Research Paper
 
Identifier Pranathi, K., Kalyani, M.B., Viraktamath, B.C. et al. Expression profiling of immature florets of IR58025A, a wild-abortive cytoplasmic male sterile line of rice and its cognate, isonuclear maintainer line, IR58025B. 3 Biotech 9, 278 (2019). https://doi.org/10.1007/s13205-019-1806-5
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http://krishi.icar.gov.in/jspui/handle/123456789/52556
 
Language English
 
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Publisher Not Available