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Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers

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Title Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers
 
Creator Norman, P.E.
Agre, P.A.
Danquah, A.
Tongoona, P.B.
Danquah, E.Y.
Koeyer, D. de
Ikeogu, Ugochukwu N.
Asiedu, R.
Asfaw, A.
 
Subject yams
dioscorea rotundata
dna
food production
paternity
ecology
 
Description White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design.
 
Date 2020
2022-05-04T11:53:45Z
2022-05-04T11:53:45Z
 
Type Journal Article
 
Identifier Norman, P.E., Agre, P.A., Danquah, A., Tongoona, P.B., Danquah, E.Y., De Koeyer, D., ... & Asfaw, A. (2020). Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers. Plants, 9(4): 527, 1-15.
2223-7747
https://hdl.handle.net/10568/119459
https://doi.org/10.3390/plants9040527
BIOTECH & PLANT BREEDING
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1-15
application/pdf
 
Publisher MDPI AG
 
Source Plants