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MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene

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Title MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene
 
Creator He, Mingliang
 
Contributor Zhang, Weibo
Wang, Shan
Ge, Ling
Cao, Xiukai
Wang, Shanhe
Yuan, Zehu
Lv, Xiaoyang
Getachew, Tesfaye
Mwacharo, Joram
Haile, Aynalem
Sun, Wei
 
Subject differentiation
hu sheep
no poverty
poverty reduction, livelihoods and jobs
mir-181a
yap1
proliferation
satellite cell
 
Description MicroRNA (miRNA) is of great importance to muscle growth and development, including
the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our
research group’s previous study, we found that miR-181a is differentially expressed in the longissimus
dorsi muscle of Hu sheep at different stages. We speculated that miR-181a may participate in the
growth and development process of Hu sheep. To understand the mechanism of miR-181a regulating
the growth and development of Hu sheep skeletal muscle, we extracted skeletal muscle satellite
cells from the longissimus dorsi muscle of 3-month-old Hu sheep fetuses and performed a series of
experiments. Our results showed that miR-181a suppressed SMSCs’ proliferation using QRT-PCR,
Western blot, CCK-8, EDU, and Flow cytometry cycle tests. In addition, QRT-PCR, Western blot,
and immunofluorescence indicated that miR-181a facilitated the differentiation of SMSCs. Then, we
used dual-luciferase reporter gene detection, QRT-PCR, and Western blot to find that the Yes1-related
transcription regulator (YAP1) is the target gene of miR-181a. Our study supplies a research basis for
understanding the regulation mechanism of miR-181a on the growth of Hu sheep skeletal muscle.
 
Date 2022-03-16
2023-01-03T21:51:22Z
2023-01-03T21:51:22Z
 
Type Journal Article
 
Identifier https://mel.cgiar.org/reporting/download/hash/a526fd8e1cdb9ac81c45394bb5822166
Mingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram Mwacharo, Aynalem Haile, Wei Sun. (16/3/2022). MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene. Genes, 13 (3).
https://hdl.handle.net/20.500.11766/67860
Open access
 
Language en
 
Rights CC-BY-4.0
 
Format PDF
 
Publisher MDPI
 
Source Genes;13,(2022)