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Immobilization kinetics of CMCase and β-glucosidase from Aspergillus niger in calcium alginate beads

Indian Agricultural Research Journals

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Title Immobilization kinetics of CMCase and β-glucosidase from Aspergillus niger in calcium alginate beads
 
Creator GARG, NEELIMA
KUMAR, DEVENDRA
YADAV, KAUSHLESH K
M, MUTHUKUMAR
 
Subject Aspergillus niger, β-glucosidase, CMCase, Immobilization, Mango peel
 
Description Carboxymethyl cellulase (CMCase) and β-glucosidase were produced by Aspergillus niger using mango peel residue as substrate and immobilized on calcium alginate beads through entrapment technique. The catalytic properties of the immobilized CMCase and β-glucosidase were compared to free enzyme. The enzyme efficiency of the immobilized CMCase and β-glucosidase were found to be 89.52 and 86.61%, respectively. Maximum immobilization efficiency was achieved on beads formed by 5% (m/v) of sodium alginate and bead size of 2 mm and 1.5 mm, for CMCase and β-glucosidase, respectively. The immobilized enzyme showed higher stability over a wider pH and temperature range. Metal ions, viz. Cu2+, Mo2+, Mn2+, Ca2+, B3+, Zn2+, Mg2+ and Fe2+ showed positive effect for immobilized β-glucosidase activity, while Mg2+ on immobilized CMCase activity. The immobilized enzymes could express 20-25% relative enzyme activity even after 4th repeated use. Immobilization improved the thermal stability over the free enzyme and affected the enzyme kinetic constants, viz. KM, Vmax and activation energy.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2015-01-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/45937
 
Source The Indian Journal of Agricultural Sciences; Vol 85, No 1 (2015)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/45937/19914
 
Rights Copyright (c) 2015 The Indian Journal of Agricultural Sciences