Record Details

Pathogenesis and expression profile of selected immune genes to experimental Edwardsiella tarda infection in iridescent shark, Pangasianodon hypophthalmus.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Pathogenesis and expression profile of selected immune genes to experimental Edwardsiella tarda infection in iridescent shark, Pangasianodon hypophthalmus.
Not Available
 
Creator F Hoque, , Dutta, R., Sawant, B., Babu, G. P., Choudhari, A. and Sundaray, J. K.
N Pawar
P Pitale
R Dutta
B Sawant
G.P Babu
A Choudhari
J. KSundaray
 
Subject Edwardsiellosis, Mortality, Necrobiotic changes, Tissue specificity, Immune gene expression
 
Description Not Available
Pathogenesis and immune gene expression of the iridescent shark catfish Pangasianodon hypophthalmus to experimental Edwardsiella tarda infection was studied. Healthy Pangasianodon were inoculated intramuscularly with a sub-lethal dose of 1.77 × 107 cells/fish of E. tarda to induce edwardsiellosis. Petechial haemorrhages, inflammation at the site of injection, vertical hanging from the water surface, swollen abdomen and anorexia were the common signs observed in challenged catfish fingerlings. The morbid catfish also exhibited typical signs of septicaemia with haemorrhages on ventral parts of the body and fin bases and congested vent prior to death. The mortality was observed 48 h post injection. The average percent morality was 42 % in fish exposed to 1.77 × 107 cells of E. tarda, whereas, only6.6 % mortality was observed in placebo control (catfish received 0.1 mL of 0.85 % sterile physiological saline) and no mortality in negative control group (catfish received no injection) over an observational period of 20 days. The kidney and liver of moribund fish demonstrated different necrobiotic changes. In kidney, cellular and cloudy swelling, degeneration of renal tubules, necrosis of glomerulus, dilation of Bowman’s space, hypertrophy, fibrosis of renal tubules and increased melano-macrophage reaction were frequently observed. In liver, disorganized hepatocytes, blood congestion and nuclear pyknosis were evident. To ascertain the host response to E. tarda infection, mRNA levels of three different classes of innate immune components viz. cytokine interleukin-1β (IL-1β), acute phase protein transferrrin and complement C3 were assessed by quantitative real-time PCR. Significant up-regulation (P 
Not Available
 
Date 2023-01-03T13:15:10Z
2023-01-03T13:15:10Z
2020-07
 
Type Journal
 
Identifier Hoque, F., Pawar, N., Pitale, P., Dutta, R., Sawant, B., Babu, G. P., Choudhari, A. and Sundaray, J. K. (2020). Pathogenesis and expression profile of selected immune genes to experimental Edwardsiella tarda infection in iridescent shark, Pangasianodon hypophthalmus. Aquaculture Reports, 17: 100371. DOI: 10.1016/j. aqrep.2020.100371.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/75313
 
Language English
 
Relation Not Available;
 
Publisher Not Available