Record Details

Effect of population size on the estimation of QTL: a test using resistance to barley stripe rust

MELSpace

View Archive Info
 
 
Field Value
 
Title Effect of population size on the estimation of QTL: a test using resistance to barley stripe rust
 
Creator Vales, Isabel
 
Contributor C.Schön, Chris
Capettini, Flavio
Chen, X.M
Corey, A. E.
Mather, D.E
Mundt, Christopher C.
Richardson, KL
Sandoval-Islas, J. S.
Utz, H. F.
Hayes, PM
 
Subject selective genotyping
selective phenotyping
Æ puccinia striiformis f. sp. hordei
 
Description The limited population sizes used in many quantitative trait locus (QTL) detection experiments can lead to underestimation of QTL number, overestimation of QTL effects, and failure to quantify QTL interactions. We used the barley/barley stripe rust pathosystem to evaluate the effect of population size on the estimation of QTL parameters. We generated a large (n=409) population of doubled haploid lines derived from the cross of two inbred lines, BCD47 and Baronesse. This population was evaluated for barley stripe rust severity in the Toluca Valley, Mexico, and in Washington State, USA, under field conditions. BCD47 was the principal donor of resistance QTL alleles, but the susceptible parent also contributed some resistance alleles. The major QTL, located on the long arm of chromosome 4H, close to the Mlo gene, accounted for up to 34% of the phenotypic variance. Subpopulations of different sizes were generated using three methods—resampling, selective genotyping, and selective phenotyping—to evaluate the effect of population size on the estimation of QTL parameters. In all cases, the number of QTL detected increased with population size. QTL with large effects were detected even in small populations, but QTL with small effects were detected only by increasing population size. Selective genotyping and/or selective phenotyping approaches could be effective strategies for reducing the costs associated with conducting QTL analysis in large populations. The method of choice will depend on the relative costs of genotyping versus phenotyping.
 
Date 2005-09-23
2023-01-04T22:06:36Z
2023-01-04T22:06:36Z
 
Type Journal Article
 
Identifier https://mel.cgiar.org/dspace/limited
Isabel Vales, Chris C. Schön, Flavio Capettini, X. M Chen, A. E. Corey, D. E Mather, Christopher C. Mundt, KL Richardson, J. S. Sandoval-Islas, H. F. Utz, PM Hayes. (23/9/2005). Effect of population size on the estimation of QTL: a test using resistance to barley stripe rust. TAG Theoretical and Applied Genetics, 111, pp. 1260-1270.
https://hdl.handle.net/20.500.11766/67873
Timeless limited access
 
Language en
 
Format PDF
 
Publisher Springer (part of Springer Nature)
 
Source TAG Theoretical and Applied Genetics;111,(2005) Pagination 1260-1270