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Bio-hardening of in-vitro raised plants of Bhagwa pomegranate (Punica granatum)

Indian Agricultural Research Journals

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Title Bio-hardening of in-vitro raised plants of Bhagwa pomegranate (Punica granatum)
 
Creator SINGH, N V
SHARMA, JYOTSANA
CHANDRA, RAM
BABU, K D
SHINDE, Y R
MUNDEWADIKAR, D M
PAL, R K
 
Subject Arbuscular mycorrhizal fungi, Aspergillus niger strain AN-27 Bio-hardening, Pomegranate
 
Description A pot culture experiment on bio-hardening of the in-vitro raised plants of Bhagwa pomegranate (Punica granatum L.) was conducted to find out the effect of two commercially available bio-formulations containing Arbuscular Mycorrhizal Fungi (AMF) and Aspergillus niger strain AN-27. Observations on root colonization, population dynamics of microbes in rhizospheric soil, growth, physiological and biochemical parameters of biohardened plants were recorded at 180 days after inoculation. Results revealed that AMF colonization in roots of in-vitro raised pomegranate plants was found at par in plants inoculated with AMF (71.12%) and AMF + Asp (65.00%) the root colonization in these two treatments was significantly higher than Contral (10.00%). Population of Aspergillus niger in the rhizospheric soil was found significantly higher in soil inoculated with Aspergillus niger strain AN-27 (6 × 104 cfu/g of soil) as compared to non-inoculated control (2 × 104 cfu/g of soil). Significant improvement in plant height (27.0% increase), shoot fresh weight (24.8% increase), root fresh (23.3% increase) and dry weight (15.7% increase) was observed in bio-hardened plants than untreated ones. Physiological processes were also significantly improved by bio-hardening as AMF and AMF + Asp treated plants registered significantly better RWC (92.34 and 91.74 %, respectively) and photosynthesis (12.69 and 12.78 μmol CO2m-2s-1, respectively) as compared to control (87.76 % and 9.07 μmol CO2m-2s-1, respectively) and only Asp treated plants (89.99% and 9.70 μmol CO2m-2s-1, respectively). AMF inoculated and AMF + Asp treated plants registered significantly higher total leaf chlorophyll (2.97 and 3.14 mg/g fresh weight, respectively) and total phenolic content (53.00 and 52.50 mg catechol equivalent/ 100 g fresh wt., respectively) than the control and only Asp treated plants.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2016-01-22
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/55252
 
Source The Indian Journal of Agricultural Sciences; Vol 86, No 1 (2016)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/55252/23230
 
Rights Copyright (c) 2016 The Indian Journal of Agricultural Sciences