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Role of genotype, explant and growth hormones on regeneration in eggplant (Solanum melongena)

Indian Agricultural Research Journals

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Title Role of genotype, explant and growth hormones on regeneration in eggplant (Solanum melongena)
 
Creator KAUR, MOHINDER
DHATT, AJMER SINGH
SANDHU, JAGDEEP SINGH
SIDHU, AMRIK SINGH
 
Subject Cotyledon; Eggplant; Hypocotyl; Leaf; Regeneration; Somatic embryogenesis
 
Description Induction of somatic embryos in cultured hypocotyl, cotyledon and leaf explants of BL 5, BR 14 and BSR 23 genotypes of eggplant (Solanum melongena) was investigated on MS medium fortified with different concentrations of IBA and BAP. The statistical analysis showed significant differences among various interactions. Cotyledon of BL 5 exhibited 89.02 and 87.35% callusing and somatic embryogenesis, respectively with fortification of MS using 1.5 mg/1 IBA + 1.0 mg/l BAP. Whereas, hypocotyl of all genotypes produced 100% callusing with poor somatic embryogenesis on all the MS media combinations (0.5–1.5 mg/l IBA + 1.0 mg/l BAP). Compact, nodular and friable cotyledonary callus of BSR 23 attained 64.86% plant regeneration on MS fortified with 2.5 mg/l BAP + 1.0 mg/l Kin + 0.2% activated charcoal, with significant differences from cotyledonary callus of BR 14 (57.33%) and BL 5 (52.83%). As a whole, the cotyledonary callus of BSR 23 exhibited somatic embryos with highest regenerative potential/P>
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-01-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2591
 
Source The Indian Journal of Agricultural Sciences; Vol 81, No 1 (2011)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/2591/713
 
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