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Genetic diversity analysis of marigold (Tagetes sp) genotypes using RAPD and ISSR markers

Indian Agricultural Research Journals

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Title Genetic diversity analysis of marigold (Tagetes sp) genotypes using RAPD and ISSR markers
 
Creator NAMITA, NAMITA
PANWAR, SAPNA
SONAH, HUMIRA
SINGH, KANWAR P
SHARMA, T R
 
Subject Cluster analysis, Genetic diversity, PCA, Polymorphism, RAPD and ISSR markers, Tagetes sp
 
Description The genetic diversity of 15 genotypes of Tagetes erecta L. (African marigold; Af/ws-1, Af/ws-2, Af/ws-3, Af/ws-4, Af/ws-5, Af/ws-6 and Af/ws-7) and Tagetes patula L. (French marigold; Fr/ws-1, Fr/ws-2, Fr/ws-3, Fr/ws-4, Fr/ws-5, Fr/ ws-6, Fr/ws-7 and Fr/ws-8) were assessed using PCR based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat) markers. Twenty three RAPD primers were used to generate polymorphism and mean per cent polymorphism was found as 57.72% ranging from 33.33% (OPF-2to   83.33% (RAPD-07). Five most informative primers (OPA-02, OPA-04, OPO-10, RAPD-07 and RAPD-18) were identified on the basis of level of polymorphism detected by individual primer. Twelve ISSR primers were also used to generate polymorphism and mean per cent polymorphism was 60.48% ranging from 40.00% (ISSR-02, ISSR-17) to 90.00% (ISSR-03). The five most informative primers (ISSR-03, ISSR-06, ISSR-12, ISSR-31 and ISSR-18) were also identified on the basis of polymorphism. The dendrogram obtained from UPGMA cluster analysis of Jaccard’s similarity values based on 23 RAPD and 12 ISSR markers grouped 15 genotypes into two clusters species wise (Tagetes erecta and Tagetes patula) which confirm the reliability of PCR based molecular markers. PCA analysis also confirmed the grouping of genotypes as they were present in same group as revealed by RAPD and ISSR markers.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-05-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/29631
 
Source The Indian Journal of Agricultural Sciences; Vol 83, No 5 (2013)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/29631/13333
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences