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Expression of messenger RNA encoding LIF receptor beta in buffalo preimplantation embryos produced in vitro

Indian Agricultural Research Journals

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Title Expression of messenger RNA encoding LIF receptor beta in buffalo preimplantation embryos produced in vitro
 
Creator ESWARI, S
SAIKUMAR, G
SHARMA, G TARU
 
Subject Buffalo; Gene expression; In vitro embryo development; LIFRb
 
Description Leukaemia inhibitory factor (LIF) is a pleotrophic cytokine from interleukin–6 family and plays an essential role during embryonic development and implantation. LIF exerts its biological effects via binding to a specific receptor system; LIF receptor beta (LIFRß) and glycoprotein 130 (gp130). While LIFRß serves as the specific binding unit for the LIF, the gp130 subunit is similarly used by other cytokines. The temporal expression pattern of genes for several growth factors and their receptors have been examined in pre-implantation buffalo embryos using RT- PCR. This study investigated the expression of buffalo LIFRß in immature, in vitro matured oocytes and pre-implantation embryos. A pool of immature oocytes, matured oocytes, 2–4 cells, 8–16 cells, morulae and blastocyst were collected and used for total RNA isolation. The transcripts encoding LIFRb were detected by employing RT-PCR. LIFRß mRNA were present during the pre-implantation stages of development from immature oocytes to the hatched blastocyst. The presence of functional LIFRb in the preimplantation embryos indicated the important role of cytokine LIF in embryo development.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-01-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2962
 
Source The Indian Journal of Animal Sciences; Vol 81, No 1 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2962/1041
 
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