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PCR based sex differentiation in guinea fowl using W chromosome specific sequences

Indian Agricultural Research Journals

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Title PCR based sex differentiation in guinea fowl using W chromosome specific sequences
 
Creator Singh, M K
Singh, H N
Gupta, Atul
Shukla, P K
Sharma, Deepak
 
Subject Guinea fowl; Multiplex PCR; Sex identification; W chromosome
 
Description In view of poor sexual bimorphism in guinea fowl, an attempt was made to develop PCR based method for sex differentiation using W chromosome specific sequence. A 370 bp sex specific fragment was amplified in female birds of guinea fowl, chicken, quail and turkey using the EE0.6 specific set of primers i.e. USP1 and USP3, while no amplification was observed in male birds. The 16S rRNA gene specific primers were used to amplify a ~590 bp fragment in both the sexes, which acted as control. A multiplex PCR assay was developed by using sex specific primers and universal 16S rRNA primers for sex differentiation in guinea fowl and other galliformes species. In all the species including guinea fowl, female showed 2 bands i.e. 370 bp female specific and 590 bp common band, while males showed only 590 bp band. These results showed the effectiveness of sex specific primers in sex differentiation in guinea fowl as well as other galliformes species.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-05-20
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/6306
 
Source The Indian Journal of Animal Sciences; Vol 81, No 6 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/6306/2518
 
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